Stromal cell-derived factor-1 from bone marrow mesenchymal stem cells protects myocardial cells

doi:10.3969/j.issn.2095-4344.2013.06.003

Abstract

Abstract:

BACKGROUND: Several studies have demonstrated that stem cells expressing CXCR4 can migrate into myocardial infarction region to improve the heart function by regenerating myocardial tissue and vessels under the gradient concentrations of stromal cell-derived factor-1.
OBJECTIVE: To investigate the protective effect of stromal cell-derived factor-1 from bone marrow mesenchymal stem cells on myocardial cells.
METHODS: Conditioned medium of bone marrow mesenchymal stem cells cultured for 2 days was collected. Under hypoxic condition, H9C2 cells were pretreated with CXCR4 inhibitor AMD3100 (5 mg/mL) or PI3-K/Akt inhibitor LY294002 (10 μM) for 1 hour. After treated with bone marrow mesenchymal stem cell conditioned medium, H9C2 cell apoptosis was analyzed by Annexin V/PI double staining methods. Expression of Akt and pAkt in H9C2 cells was analyzed by Western blotting. Expression of stromal cell-derived factor-1 was analyzed by RT-PCR.
RESULTS AND CONCLUSION: RT-PCR results showed that bone marrow mesenchymal stem cells expressed stromal cell-derived factor-1. Western blotting results showed that bone marrow mesenchymal stem cell conditioned medium increased pAkt protein level in H9C2 cells. Annexin V/PI analysis showed that bone marrow mesenchymal stem cell conditioned medium significantly decreased apoptosis of H9C2 cells induced by hypoxia/reoxygenation and this anti-apoptotic effect could be blocked by CXCR4 inhibitor AMD3100 or PI3-K/Akt inhibitor LY294002. Stromal cell-derived factor-1 from mesenchymal stem cells plays an important role in the protection of cardiomyocytes through PI3-K/Akt signal pathway.

Key words: stem cells, bone marrow-derived stem cells, bone marrow mesenchymal stem cells, H9C2, myocardial cells, myocardial infarction, stromal cell-derived factor-1, apoptosis, PI3-K/Akt, National Natural Science Foundation of China, stem cell photographs-containing paper

CLC Number:

R394.2

Mao Hong-bo, Zou Sai, Tang Jun-ming, Yang Jian-ye, Wang Jia-ning, Kong Xia, Guo Ling-yun, Zheng Fei, Zhang Lei, Huang Yong-zhang. Stromal cell-derived factor-1 from bone marrow mesenchymal stem cells protects myocardial cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 963-968.

Figures/Tables 5

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