Reconstruction of tissue-engineered heart valve with human bone marrow stromal stem cells in vitro

doi:10.3969/j.issn.2095-4344.2013.06.002

Abstract

Abstract:

BACKGROUND: Nowadays, mechanical or biological valve recipients used in the clinic are still at the risk of infection, hemorrhage, thrombosis and reoperation owing to valve stenosis. Tissue-engineered heart valve with biological activity can overcome the disadvantages above. While, the optimal choice of scaffolds and seeding cells remains disputable.
OBJECTIVE: To explore the feasibility to construct tissue-engineered heart valve with acellularized porcine aortic valve scaffold and human bone marrow stromal stem cells in vitro.
METHODS: The porcine aortic valves were decellularized with the detergent and enzymatic extraction process to remove the cellular components. Human bone marrow stromal stem cells were aspirated from sternum of the patients with simple congenital heart malformation, and then the cells were seeded on the acellularized porcine aortic valve scaffold and cultured for 5 days.
RESULTS AND CONCLUSION: Flow cytometry identified that the characteristics of surface antigen of the inoculated seed cells were in line with those of human bone marrow stromal stem cells. Light microscopy and electron microscopy confirmed that the cellular components in the porcine aortic valves could be removed to obtain the complete acellular fiber mesh stent. There was no significant difference in biomechanical property between before and after acellularization. The human bone marrow stromal stem cells implanted on the acellularized porcine aortic valve scaffold could form a continuous cell layer on the surfaces of the scaffold. The inoculated bone marrow stromal stem cells could be differentiated into fibroblasts. The implantation of human bone marrow stromal stem cells on the acellularized porcine aortic valve scaffold can construct the tissue-engineered heart valve.

Key words: stem cells, bone marrow-derived stem cells, acellularized porcine aortic valve scaffold, acellular, aortic valve, porcine, scaffold, bone marrow stromal stem cells, human, heart valve, tissue engineering, regenerative medicine, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R349.2

Kang Kai1, Qu Hui2, Tang Ji-quan, Jia Zhi-bo, Zhang Yu-nan, Zhang Chun-feng, Wu Hua, Li Ren-ke, Jiang Shu-lin. Reconstruction of tissue-engineered heart valve with human bone marrow stromal stem cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 957-962.

Figures/Tables 4

References

[1] Dainese L, Guarino A, Burba I,et al. Heart valve engineering: decellularized aortic homograft seeded with human cardiac stromal cells. J Heart Valve Dis. 2012;21(1):125-134.

[2] Jordan JE, Williams JK, Lee SJ, et al. Bioengineered self-seeding heart valves. J Thorac Cardiovasc Surg. 2012; 143(1):201-208.

[3] Weber B, Scherman J, Emmert MY,et al. Injectable living marrow stromal cell-based autologous tissue engineered heart valves: first experiences with a one-step intervention in primates. Eur Heart J. 2011;32(22):2830-2840.

[4] Kang K,Tan Z,Jiang SL,et al. Zhongguo Xiong Xin Xueguan Waike Zazhi. 2005;12(5): 339-342. 康凯,谭震,蒋树林,等.去细胞猪主动脉瓣支架及兔骨髓干细胞构建组织工程心脏瓣膜的初步研究[J].中国胸心血管外科临床杂志,2005,12(5): 339-342.

[5] Zhao GR, Zhang HM, Ye TX,et al. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B. Food Chem Toxicol. 2008;46(1):73-81.

[6] Ma JB,Zhong H,Han SX,et al. Zhonghua Xiong Xin Xueguan Waike Zazhi. 2009 ;25(4): 257-259. 马金本,钟竑,韩绍先,等.叠氮钠对脱细胞组织工程心脏瓣膜形态学及生物力学的影响[J].中华胸心血管外科杂志,2009 ,25(4): 257-259.

[7] Xie JQ,Wei XF,Yi DH. Zhonghua Xiong Xin Xueguan Waike Zazhi. 2009;25(5): 289-291. 谢甲琦,魏旭峰,易定华.心脏瓣膜的结构——构建组织工程心脏瓣膜的蓝图[J].中华胸心血管外科杂志,2009,25(5): 289-291.

[8] Cebotari S, Lichtenberg A, Tudorache I,et al. Clinical application of tissue engineered human heart valves using autologous progenitor cells. Circulation. 2006;114(1 Suppl): I132-137.

[9] Weber B, Emmert MY, Behr L,et al. Prenatally engineered autologous amniotic fluid stem cell-based heart valves in the fetal circulation. Biomaterials. 2012;33(16):4031-4043.

[10] Prockop DJ. Marrow stromal cells as stem cells for nonhematopoietic tissues.Science. 1997;276(5309):71-74.

[11] Xiao YF, Min JY, Morgan JP. Immunosuppression and xenotransplantation of cells for cardiac repair. Ann Thorac Surg. 2004;77(2):737-744.

[12] Roy A, Brand NJ, Yacoub MH. Molecular characterization of interstitial cells isolated from human heart valves. J Heart Valve Dis. 2000;9(3):459-465.

[13] Oswald J, Boxberger S, Jørgensen B,et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells. 2004;22(3):377-384.

[14] Beitnes JO, Lunde K, Brinchmann JE,et al. Stem cells for cardiac repair in acute myocardial infarction.Expert Rev Cardiovasc Ther. 2011;9(8):1015-1025.

[15] Halimu•Kelimu,Wang L,Qi H. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(34):6432-6435. 哈里木•克里木,王磊,齐海.组织工程心脏瓣膜支架材料的选择与应用[J].中国组织工程研究与临床康复,2011,15(34): 6432-6435.

[16] Zhou JL,Zou MH,Hu XJ,et al. Zhongguo Linchuang Yishi Zazhi. 2011;5(10):2890-2895. 周建良,邹明晖,胡行健,等.血管内皮细胞生长因子修饰的聚乙二醇化去细胞瓣构建心脏瓣膜复合支架[J].中华临床医师杂志:电子版, 2011,5(10): 2890-2895.

[17] Flanagan TC, Cornelissen C, Koch S,et al. The in vitro development of autologous fibrin-based tissue-engineered heart valves through optimised dynamic conditioning. Biomaterials. 2007;28(23):3388-3397.

[18] Wang P,Li C,Tan L,et al. Shandong Yiyao. 2009;49(26): 34-35. 王鹏,李超,谈磊,等.三种去细胞组织工程异种瓣膜支架制备方法比较[J].山东医药,2009,49(26):34-35.

[19] Engelmayr GC Jr, Sales VL, Mayer JE Jr,et al. Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Biomaterials. 2006;27(36): 6083-6095.

[20] Syedain ZH, Weinberg JS, Tranquillo RT. Cyclic distension of fibrin-based tissue constructs: evidence of adaptation during growth of engineered connective tissue. Proc Natl Acad Sci U S A. 2008;105(18):6537-6742.