Construction of acellular muscle bioscaffolds using three approaches: Histological and biomechanical comparison

doi:10.3969/j.issn.2095-4344.2013.03.014

Abstract

Abstract:

BACKGROUND: The methods of constructing acellular muscle bioscaffolds include chemical method, frozen/thawing method and freezing method.OBJECTIVE: To study the histological and biomechanical differences among the acellular muscle bioscaffolds prepared using chemical method, frozen/thawing method and reformed chemical method.
METHODS: Totally 36 segments of the erector spinae from six Sprague-Dawley rats were randomized into three groups by different preparation methods (n=12): chemical method group (group A), frozen/thawing group (group B) and reformed chemical method group (group C). After sterilization and seeding of bone mesenchymal stem cells, the scaffolds from three groups were compared on histology and biomechanics.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed the internal structure was in parallel rows in the three group, but the continuity of scaffold structure in group A was less than groups B and C. Masson staining confirmed essential component of scaffolds was collagen fibers in the three group and some residual muscle fibers in group B with asymmetry clearance. The number of living seed cells labeled by fluorescence in group B was significantly less than that in groups A and C at 7 days (P < 0.01) and less than that in group C at 14 days (P < 0.05). Lots of cells attaching to each group scaffolds were flourished confirmed by scanning electron microscope. Group B exceeded group A in the maximum load of scaffold (P < 0.05) but not group C. There was no difference among three groups in modulus of elasticity. Porosity of group B was lower than that of groups A and C (P < 0.05). These findings indicate that the reformed chemical method is more ideal, which can balance the histology and biomechanics of acellular muscle bioscaffolds, remove muscle cells more thoroughly and maintain more extracellular matrix.

Key words: biomaterials, material mechanics and surface modification, acellular muscle bioscaffolds, bone marrow mesenchymal stem cells, chemical method, scaffold construction, histology, biomechanics, frozen/thawing method, other grants-supported paper, biomaterial photographs-containing paper

CLC Number:

R318

Wei Xiang-ke, Wen Yi-min, Zhang Tao, Li Han. Construction of acellular muscle bioscaffolds using three approaches: Histological and biomechanical comparison[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 465-471.

Figures/Tables 7

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