Influence of soy isoflavone tincture on wound healing of deep partial-thickness scald in mice

doi:10.3969/j.issn.2095-4344.2013.02.014

Abstract

Abstract:

BACKGROUND: Estrogen has a role to promote wound healing, and combination of soy isoflavones and estrogen receptor presents an obvious estrogen-like effect.
OBJECTIVE: To explore the preparation of soy isoflavone tincture and to study its influence on wound healing in scalded mice.
METHODS : Soy isoflavone tincture was prepared by cold dipping and the most effective concentration was
screened. At the beginning of the experiment, all of the mice were used to prepare deep partial-thickness scald models and then randomly divided into soy isoflavone tincture (0.100, 0.361, 1.000, 3.610 g/L), solvent control (75% ethanol) and blank (without treatment) groups. From the 3rd day on, nonhealing rates of wound were assessed every other day. Simultaneously, the histological and morphological changes were observed and analyzed at days 3, 9, 14 after modeling.
RESULTS AND CONCLUSION: The most effective concentration of soybean isoflavone tincture was 3.61 g/L. On days 5, 7, 9, 11, 13 after injury, the nonhealing rate of wound in the soybean isoflavone tincture group was significantly less than that of the blank group (P < 0.05), as well as that of the solvent control group (P < 0.05). On day 9 post scalded, the histomorphology displayed that there were many more fibroblasts in the granulation tissue in the soy isoflavone tincture group than those of the blank group, also than those of the solvent control group. On day 14 post scalded, the histomorphology showed that the epithelial layer in the soy isoflavone tincture group was thicker than that of the other two groups. It is a viable method that soy isoflavone tincture is prepared in 75% ethanol solution, and its concentration of 3.61 g/L can significantly accelerate the wound healing in scalded mice by local application.

Key words: tissue construction, skin tissue construction, soy isoflavone tincture, wound healing, scald models, mice, provincial grants-supported paper, tissue construction photographs-containing paper

CLC Number:

R318

Zhang Li, Chen Juan, Su Wei-sheng, Huang Jia-jun. Influence of soy isoflavone tincture on wound healing of deep partial-thickness scald in mice[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 264-269.

Figures/Tables 6

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