Recombinant green fluorescent protein plasmids expressing human telomerase reverse transcriptase transfect normal nucleus pulposus cells in the intervertebral disc

doi:10.3969/j.issn.2095-4344.2013.02.013

Abstract

Abstract:

BACKGROUND: Nucleus pulposus cells are difficult to be isolated and cultured from the intervertebral disc, and the cells age rapidly. An urgent standard cell line is required for experimental research.
OBJECTIVE: To investigate the construction of recombinant green fluorescent protein plasmid expressing human telomerase reverse transcriptase and to assess the feasibility of constructing immortalized human nucleus pulposus cell line.
METHODS: Target gene cloning, target gene sequencing in eukaryotic expression plasmids, construction of eukaryotic expression plasmids expressing target genes, and transfection of human telomerase reverse transcriptase were performed in order.
RESULTS AND CONCLUSION: Nucleus pulposus cells transfected with recombinant green fluorescent protein plasmid expressing human telomerase reverse transcriptase have a stable expression. It is anticipated that human telomerase reverse transcriptase can be useful for creating the immortalized nucleus pulposus cells.

Key words: tissue construction, spinal cord tissue construction, nucleus pulposus cells, human telomerase reverse transcriptase gene, DH5&alpha, competent Escherichia coli, transfection, immortalized, target gene, nucleus pulposus cells transfection in degenerative disc diseases, provincial fund, tissue construction photographs-containing paper

CLC Number:

R318

Wu Jin-feng, Ye Dong-ping, Dai Li-bing, Liang Wei-guo. Recombinant green fluorescent protein plasmids expressing human telomerase reverse transcriptase transfect normal nucleus pulposus cells in the intervertebral disc[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 259-263.

Figures/Tables 4

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