Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (19): 3031-3038.doi: 10.3969/j.issn.2095-4344.3535
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Zhang Kai1, 2, Zhang Xiaobo1, 2, Shi Jintao1, 2, Wang Keping3, Zhou Haiyu1, 2, 3
Received:
2020-07-16
Revised:
2020-07-21
Accepted:
2020-08-29
Online:
2021-07-09
Published:
2021-01-13
Contact:
Zhou Haiyu, MD, Chief physician, Master’s supervisor, Department of Orthopedics, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China; Key Laboratory of Bone and Joint Disease Research of Gansu Province, Lanzhou 730030, Gansu Province, China; Department of Orthopedics, Xigu Hospital of Lanzhou University Second Hospital, Lanzhou 730060, Gansu Province, China
About author:
Zhang Kai, Master candidate, Department of Orthopedics, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China; Key Laboratory of Bone and Joint Disease Research of Gansu Province, Lanzhou 730030, Gansu Province, China
Supported by:
CLC Number:
Zhang Kai, Zhang Xiaobo, Shi Jintao, Wang Keping, Zhou Haiyu. Mesenchymal stem cells for treatment of intervertebral disc degeneration: a bibliometric and visualization analysis based on Web of Science database[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3031-3038.
2.3 间充质干细胞治疗椎间盘退行性疾病文献的作者合作情况 对领域内作者合作网络进行分析,见图2,共有206位作者被纳入,发文量≥3篇共有70位。图中圆点大小代表作者在本领域的发文量,连线粗细代表作者间合作的紧密程度。结果显示,该领域存在多个研究团体,①以Daisuke Sakai(日本)的发文量(22篇)和中心性(0.03)最高,研究内容是间充质干细胞移植对退变椎间盘的再生作用[12-14],他与Joji Mochida (日本)、Sibylle Grad(瑞士)、Mauro Alini(瑞士)和Lorin M Benneker(瑞士)等作者之间展开合作,共同构成该领域最大的作者合作研究团体;②第2大团体是以Judith A Hoyland(英国)为中心,包括Stephen M Richards(英国)和Antony J Freemont(英国)等主要研究者,研究内容是诱导间充质干细胞分化促进椎间盘细胞再生[15-17];③第3大团体是以Makarand V Risbud(美国)为中心所组成的,他们的研究方向为间充质干细胞向髓核细胞分化重建受损退化的椎间盘[18-20];④第4大团体是由Qixin Chen(中国)、Fangcai Li(中国)和Chenzhen Liang(中国)等组成,他们的研究方向为微环境改变影响间充质干细胞对椎间盘的修复再生[21-22],但他们主要以团队内合作为主,团体间的合作较少。除此之外,也有个别发文较多的作者,如Yue Zhou(中国)、Zengwu Shao(中国)和Dawn M Elliott(美国)等,他们常是由个人组成的研究小团队,与其他研究者没有开展合作关系。 "
2.4 间充质干细胞治疗椎间盘退行性疾病文献的国家(地区)和机构 对该领域国家(地区)和机构进行分析,见图3,共有125个国家(地区)和机构被纳入,发文最多的国家(地区)是中国(195)、美国(187)、瑞士(58)、德国(42)和日本(41);发文最多的机构是东海大学(29)、伯尔尼大学(25)、浙江大学(22)、达沃斯AO研究所(22)和中国香港大学(20);结果显示,该领域的研究以中国最多,其次为美国、瑞士、德国和日本等国家,而来自加拿大、法国、美国、日本和英格兰的机构之间合作最为紧密,中国虽是发文量最多的国家,但是在该领域研究起步较晚,且缺乏国家间的合作,主要通过中国香港大学与新加坡等少数国家展开合作,研究内容为间充质干细胞减少椎间盘纤维化[23]、胶原联合间充质干细胞促进椎间盘再生等[24-25]。 "
2.5 间充质干细胞治疗椎间盘退行性疾病文献的期刊共被引情况 文章对该领域期刊进行共被引分析,共有154个期刊被纳入,其中被引频次最高的期刊是《Spine》(576次)、《Biomaterials》(440次)、《Eur Spine J》(432次)、《J Orthop Res》(409次)和《Spine J》(373次)。中心性值最高的期刊是《J Bone Miner Res》(0.37),《Arthritis Rheum-US》(0.36),《Bone》(0.32),《Proc Natl Acad Sci USA》(0.27)和《J Rheumatol》(0.27),见表2。期刊主要集中在骨、脊柱及生物材料方向,其中发文量最多的是《Spine》,它所发表的文章代表了该领域近年来的研究方向。 "
2.6 间充质干细胞治疗椎间盘退行性疾病文献的共被引分析 高被引文献反映了该领域中某个重要的研究成果、是该领域研究的基础,而中心性高的文献代表了本领域的关键文献。Sigma值是基于中心性和突现性计算得到的,中心性和突现性越高的节点,其Sigma值也越高,绝大多数的Sigma值是1.00,表示结构上和引文变化中都非常重要。通过对数据进行文献共被引分析,形成了452篇文献的共被引网络,归纳出共被引频次、中心性和Sigma值最高的前5名文献,见表3-5。通过对这些文献的回顾能加深对这个领域研究逻辑和理论基础的理解,将该领域研究主要内容总结为以下几个方面:间充质干细胞植入促进椎间盘细胞修复再 生[14, 26-27]、干细胞与椎间盘疾病的机制研究[15, 28-29, 32-34],以及间充质干细胞结合组织工程技术治疗椎间盘疾病 等[30-31]。 "
2.7 间充质干细胞治疗椎间盘退行性疾病文献的关键词特征 对关键词进行共现分析,有助于理解该领域的发展历史、研究重点及预测未来的发展趋势。在软件中选择Pathfinder算法结合Pruning the merged networks对整体网络进行剪切,对文献的关键词进行分析,得到206个关键词的共现图谱。出现频次的关键词是间充质干细胞(348次)、椎间盘(272次)、体外(218次)、分化(195次)和髓核(162次);中心性值最高的关键词是椎间盘退变(0.23)、间充质干细胞(0.20)、基因表达(0.17)、修复(0.17)和髓核细胞(0.17)。频次的高低代表该领域的研究热点词,而分析它们的中心性有助于揭示研究热点之间的突变或转化关系。 2.7.1 聚类图谱 采用LLR算法(此法标签合理且重复较少)对关键词共现图谱进行聚类分析(图5),形成了模块值Q为0.693 9>0.3(表明聚类结构显著)、平均轮廓值S为0.889 3>0.5(说明聚类结果合理且关联性强)的关键词聚类图谱[35],包含有10个具有代表性的聚类标签:#0共培养(coculture)、#1组织工程(tissue engineering)、#2椎间盘再生(intervertebral disc regeneration)、 #3富血小板血浆(platelet-rich plasma)、#4去细胞化(decellularization)、#5关节软骨(articular cartilage)、#6细胞力学性能(cellular mechanical properties)、#7机制(mechanism)、#8生物活性支架(bioactive scaffold)和#9脊索细胞 (notochordal cell)。表6总结了各聚类标签的特征及主要关键词,其中大小代表各聚类标签的规模大小即包含文献的数量,平均引用年限代表该聚类文献发表年份的中间值,而轮廓值S常作为评价图谱绘制效果的依据,一般S值>0.5代表此聚类合理、S值>0.7代表此聚类结果令人信服[36]。 "
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