Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (2): 254-258.doi: 10.3969/j.issn.2095-4344.2013.02.012

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Paeoniflorin accelerates annulus fibrosus cells proliferation

Chen Shao-qing1, 2, Lin Jian-ping1, 3, Wang Shi-zhong1, 2, Hong Yu1, Chen Le-chun2   

  1. 1 Fujian University of Traditional Chinese Medicine, Fuzhou  350003, Fujian Province, China
    2 Key Laboratory of Orthopedics and Exercise Rehabilitation of Ministry of Education, Fuzhou  350003, Fujian Province, China
    3 Fujian Rehabilitation Laboratory of Motor Function, Fuzhou  350003, Fujian Province, China
  • Received:2012-06-27 Revised:2012-07-28 Online:2013-01-08 Published:2013-01-08
  • Contact: Wang Shi-zhong, Doctor, Professor, Chief physician, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China; Key Laboratory of Orthopaedics and Exercise Rehabilitation of Ministry of Education, Fuzhou 350003, Fujian Province, China
  • About author:Chen Shao-qing★, Master, Assistant teacher, Physician, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China; Key Laboratory of Orthopaedics and Exercise Rehabilitation of Ministry of Education, Fuzhou 350003, Fujian Province, China chenshaoqd@163.com
  • Supported by:

    Supported by: the National Natural Science Foundation of China, No. 30973764*; Fujian Joint Research Project of Health Education, No. WKJ2008-2-48*; International Cooperation Projects of Fujian Provincial Department of Science and Technology, No. 2009I0010

Abstract:

BACKGROUND: Chinese herbaceous peony has been usually used in the treatment of cervical spondylosis in a frequency of over 30%. Paeoniflorin, known as one of the main monomers, has cell-protective abilities against inflammation and apoptosis. Despite these compelling observations, whether paeoniflorin protects annulus fibrosus cells is not reported.
OBJECTIVE: To explore the proliferative and protective effects of paeoniflorin on intervertebral disc annulus fibrosus cells.
METHODS: Annulus fibrosus cells derived from the intervertebral disc were digested with trypsin and collagenase type Ⅱ for the further culture in vitro. The third-passage annulus fibrosus cells were selected to establish an apoptosis model in response to Fas ligand (20 μg/L) and were interfered with paeoniflorin. The appropriate concentration of paeoniflorin was detected by the method of MTT. Apoptotic rate was evaluated with Annexin V/FITC double staining.
RESULTS AND CONCLUSION: The 20.8 and 2.08 μmol/L paeoniflorin significantly promoted the proliferation of annulus fibrosus cells. The apoptotic rate of annulus fibrosus cells in vitro was significantly increased after induction with the 20 μg/L Fas ligand. 20.8 μmol/L paeoniflorin could protect annulus fibrosus cells against Fas ligand-induced apoptosis.

Key words: cervical spondylosis, tissue construction, paeoniflorin, FasL, annulus fibrosus cells, apoptosis, proliferation, cytoprotection, disc degeneration, National Natural Science Foundation of China, tissue construction phothograhs-containing paper

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