Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5097-5102.doi: 10.3969/j.issn.2095-4344.2012.27.030

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Effect of quality and quantity of blood taken from unilateral and bilateral iliac on the concentration of bone marrow mononuclear cells and the efficacy of bone marrow mononuclear cells transplantation for the treatment of early femoral head necrosis

Shi Shao-hui1, Li Zi-rong2, Sun Wei2, Wang Bai-liang2, Xu Shu-qing3, Wang Ran-dong1   

  1. 1Department of Joint Trauma Surgery, Aviation General Hospital, Beijing 100029, China;
    2Center for Osteonecrosis & Joint-preserving Reconstruction, Beijing 100029, China;
    3Clinical Research Institute of China-Japan Relationship Hospital, Beijing 100029, China
  • Received:2011-11-23 Revised:2011-12-04 Online:2012-07-01 Published:2013-11-01
  • About author:Shi Shao-hui☆, M.D., Attending physician, Department of Joint Trauma Surgery, Aviation General Hospital, Beijing 100029, China ssh_snake@yahoo.com.cn

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Abstract:

BACKGROUND: Whether the quality and quantity of blood taken from unilateral and bilateral iliac would affect the concentration of bone marrow mononuclear cells?
OBJECTIVE: To observe the efficacy of mononuclear cells concentration on early femoral head necrosis treated by autologous bone marrow mononuclear cells transplantation.
METHODS: The clinical data of 120 patients of 168 hips with femoral head necrosis were retrospectively analyzed, and all the patients were processed with core decompression plus autologous mononuclear cells transplantation. This study included 27 cases (39 hips), who were taken blood about 200 mL from the unilateral iliac, 29 cases (40 hips), who were taken blood about 300 mL from the unilateral iliac, 30 cases (42 hips), who were taken blood about 200 mL from the bilateral iliac, 34 cases (47 hips), who were taken blood about 300 mL from the bilateral iliac. After the blood was taken from lilac, the bone marrow blood gradient centrifugation was performed by cell segregating, and the mononuclear cells count was observed before and after separation; the mononuclear cells were injected into the bone necrosis area after core decompression. All the patients were followed-up for 12-36 months, and the CT imaging and the Harris score of the hips in the patients before and after treatment were observed.
RESULTS AND CONCLUSION: There was no significant difference of the mononuclear cells count between subgroup of 200 mL and 300 mL taken from unilateral and bilateral iliac (P > 0.05), but the mononuclear cells count taken from the bilateral iliac was significantly higher than that taken from the unilateral iliac (P < 0.01). There was no significant difference of the Harris score between groups before transplantation (P > 0.05), and compared with before transplantation, the Harris score and the hip fineness rate was significantly increased after transplantation (P < 0.05, P < 0.01). In short, core decompression plus autologous bone marrow mononuclear cell transplantation is an easy and safe method for the treatment of early femoral head necrosis. The concentration of mononuclear cells after centrifugation when taken from bilateral iliac is significantly higher than that taken from unilateral iliac, and the better follow-up the higher concentration.

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