Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5046-2051.doi: 10.3969/j.issn.2095-4344.2012.27.020

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Effect of stromal cell derived factor-1α preconditioning on migration of rat bone marrow mesenchymal stem cells

Shu Bo1, Liu Zhi-jiang2, Fan Fang1   

  1. 1Department of Biochemistry and Molecular Biology of Zunyi Medical College, Zunyi 563003, Guizhou Province, China;
    2Department of Cardiology, First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Received:2012-03-01 Revised:2012-04-10 Online:2012-07-01 Published:2013-11-01

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Abstract:

BACKGROUND: During transplantation of bone marrow mesenchymal stem cells (BMSCs), only a few BMSCs can migrate into injured tissue. Therefore, how to increase the number of migrated BMSCs is a key to BMSC transplantation.
OBJECTIVE: To investigate the effect of stromal cell derived factor-1α (SDF-1α) preconditioning on the migration of rat BMSCs.
METHODS: BMSCs were in vitro isolated, cultured, and passaged. Using Transwell inserts technique, the effects of SDF-1α at different concentrations on the migration of BMSCs were observed and the optimal concentration of SDF-1α was determined. After preconditioned by SDF-1α, SDF-1α+AMD3100 and SDF-1α+LY294002 respectively, the migration of BMSCs was observed, and the effect of SDF-1α preconditioning on mRNA and protein level of chemokine receptor CXCR 4 in BMSCs and AKT phosphorylation were investigated.
RESULTS AND CONCLUSION: Incubation of 0.2 mg/L SDF-1α for 10 hours could produce the optimal migration of BMSCs. The role of SDF-1α in inducing the migration of BMSCs increased with the increasing concentration of SDF-1α used for 6 hours of preconditioning. AMD3100 and LY294002 could reverse the effect of SDF-1α preconditioning. The chemokine receptor CXCR 4 expression and Akt phosphorylation were increased after SDF-1α preconditioning. SDF-1α preconditioning can enhance the migration of BMSCs through increasing the expression of chemokine receptor CXCR 4. Akt signaling pathway is correlated with this migration.

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