Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 4996-5000.doi: 10.3969/j.issn.2095-4344.2012.27.011

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Rapid accumulation of autologous adipose-derived stem cells promotes early vascularization of implanted allogeneic bone

Yin Jian1, Yang Yi1, Yang Xiao-feng2, Cao Xin1, Deng Wei1, Deng Qiang1, Chai Li-ming2, Jin Ge-le1   

  1. 1First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China;
    2People’s Hospital of Xinjiang Uyghur Autonomous Region, Urumqi 830001, Xinjiang Uyghur Autonomous Region, China
  • Received:2012-03-05 Revised:2012-04-20 Online:2012-07-01 Published:2013-11-01
  • Contact: Jin Ge-le, M.D., Doctoral supervisor, Chief physician, Professor, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China jingl81@yahoo.com.cn

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Abstract:

BACKGROUND: It is not yet available to construct tissue-engineered bone quickly for repair of bone defects in the clinic, and early vascularization of the implants remains to be paid increasing attention.
OBJECTIVE: The rapidly accumulated autologous adipose-derived stem cells were directly placed around the implants to investigate the effects on early vascularization of implants using positron emission tomography PET/CT and to search a method to rapidly construct tissue-engineered bone in the clinic.
METHODS: Forty-five male New Zealand rabbits were randomly and evenly divided into three groups. These groups were implanted respectively in the lumbar 5,6 intertransverse space with allograft iliac bone (allograft group), allograft iliac bone composited with rapidly accumulated adipose-derived stem cells (compound group), and autologous iliac bone (autograft group). Each group was injected with 18F-NaF at 1, 3, 5 weeks post-surgery and detected by positron emission tomography PET/CT to contrast the standard uptake value.
RESULTS AND CONCLUSION: At 1, 3, 5 weeks post-surgery, the distribution of radioactivity in the middle-segment of allogeneic bone was low, with poor improvement in blood supply and metabolism. The standard uptake value of 18F-NaF in the autograft group and compound group was significantly higher than that in the allograft group respectively (P < 0.05). It is demonstrated that rapid accumulation of autologous adipose-derived stem cells may promote early vascularization of allogeneic bone and improve the blood supply and metabolism in the implanted area. It is also a novel idea to rapidly construct tissue-engineered bone in the clinic.

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