Abstract:

BACKGROUND: Intramyocardial transplantation of adipose-derived stem cells using biological technique for repair of injured myocardial tissue is a gradually increasing research field of cardiovascular disease. There has been no report describing cardiac-specific gene expression in cardiomyocytes induce-differentiated by rabbit adipose-derived stem cells.
OBJECTIVE: To investigate cardiac-specific gene expression in cardiomyocytes differentiated by rabbit adipose-derived stem cells under 5-azacytidine induction.
METHODS: Passage 2 adipose-derived stem cells were treated with 2.5, 5, 10, 20, 40, 80 μmo1/L 5- azacytidine for 24 hours and cultured with complete medium without 5-azacytidine. Four weeks later, cellular growth and morphological changes were observed. At the first, second, third and fourth weeks, the mRNA expression of atrial natriuretic peptide, brain natriuretic peptide and α-skeletal actin in adipose-derived stem cells was detected by RT-PCT.
RESULTS AND CONCLUSION: After 5-azacytidine induction for 1 week, the majority of cells were arranged in parallel, with increased soma, and the proportion of shuttle-shaped cells was decreased, in particular in the 40, 80 μmol/L 5- azacytidine groups. At the fourth week, cell refraction was attenuated, cell activity was weakened, and more cells died in the 40, 80 μmol/L 5-azacytidine groups. The mRNA expression of atrial natriuretic peptide, brain natriuretic peptide and α-skeletal actin in adipose-derived stem cells increased gradually. The mRNA expression of atrial natriuretic peptide, brain natriuretic peptide and α-skeletal actin in the 10 μmol/L 5-azacytidine group was significantly higher than that in the other groups (P < 0.05). 5-azacytidine induced rabbit adipose-derived stem cells show cardiac-specific gene expression.