Abstract:

BACKGROUND: Bone marrow-derived multipotent adult progenitor cells have stronger differentiation and expansion potential than bone marrow mesenchymal stem cells and exhibit the multiple differentiation potential of chondroblast-like cells. But the reports on the differentiation into chondroblast-like cells are rare.
OBJECTIVE: To study the culture and characteristics of bone marrow-derived multipotent adult progenitor cells and the potential to differentiate into chondroblast-like cells.
METHODS: The bone marrow-derived multipotent adult progenitor cells were cultured in vitro, and then the morphology and the growth conditions were observed and identified. The differentiation medium was used to introduce the bone marrow-derived multipotent adult progenitor cells to differentiate into chondroblast-like cells.
RESULTS AND CONCLUSION: Observation under the light microscope showed that bone marrow-derived multipotent adult progenitor cells were large and grew in long polygonal with several slender pseudopodia, the cells were with large nucleus and affluent cytoplasma and had strong proliferation ability; the expression of transcription factor 4 and stage-specific embryonic antigen 1 could be seen, the expressions of T-cell surface adhesion molecules of CD44, CD45 and CD133 and major organizations compatibility antigen systems Ⅱ were not shown. After induction and differentiation, toluidine blue stained differentiated cells showed metachromasia and could express collagen type Ⅱ. The bone marrow-derived multipotent adult progenitor cells were successfully cultured in vitro, and the bone marrow-derived multipotent adult progenitor cells could differentiate into the chondroblast-like cells in a certain induction conditions.