Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 4963-4967.doi: 10.3969/j.issn.2095-4344.2012.27.005

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Effects of tissue homogenate from injured liver on induced differentiation of bone marrow mesenchymal stem ce

Hou Jun-xia1, Mei Zhe-chuan1, Jiang Ming-de3   

  1. 1Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China;
    2Department of Gastroenterology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China
  • Received:2012-03-01 Revised:2012-04-24 Online:2012-07-01 Published:2013-11-01
  • Contact: Mei Zhe-chuan, Master, Master’s supervisor, Professor, Chief physician, Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China meizhechuan@21cn.com

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Abstract:

BACKGROUND: Whether bone marrow mesenchymal stem cells can differentiate into hepatocytes is closely related to microenvironment.
OBJECTIVE: To explore the effects of the acute alcoholic liver injury tissue homogenate on induced differentiation of bone marrow mesenchymal stem cells.
METHODS: BMSCs were isolated from Sprague-Dawley rats, cultured, purified and identified in vitro. Rat models of alcohol-induced acute liver injury were prepared. Tissue homogenate of normal and injured liver was used to induce differentiation of bone marrow mesenchymal stem cells. The α-fetoprotein and albumin expression was detected at 0, 3, 7, 10, 14, and 21 days using RT-PCR and western blot methods, respectively.
RESULTS AND CONCLUSION: After induced by tissue homogenate of normal and injured liver, bone marrow mesenchymal stem cells exhibit epithelioid change. After induced by each tissue homogenate, α-fetoprotein expression was detected from the 3rd day, peaked at about 7 days, decreased thereafter, and exhibited a very low level by 14 days; albumin expression was detected from the 7th day, and the expression level was gradually increased with time going. The mRNA and protein expression level was consistent. These findings demonstrate that hepatocytes gradually developed till mature. There was no significant difference in α-fetoprotein or albumin expression after induced by tissue homogenate of normal and injured liver (P > 0.05). The liver tissue homogenates of acute alcoholic liver injury can induce bone marrow mesenchymal stem cells to differentiate into hepatocytes, with similar effects to normal liver tissue homogenate.

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