Expression Foxp3 and bone morphogenetic protein 7 in rat models of carotid artery balloon injury after treated by total glucosides of paeony

doi:10.3969/j.issn.2095-4344.1940

Abstract

Abstract:

BACKGROUND: Restenosis after percutaneous coronary affects the outcome of surgery. Total glucosides of paeony has been shown to regulate autoimmune response by several pathways, and can inhibit mononuclear macrophage secreting inflammatory cytokines, eliminate oxygen free radicals, affect cell proliferation and roles of anti-inflammation, analgesia and anti-stress.
OBJECTIVE: To observe the changes in Foxp3 and bone morphogenetic protein 7 after carotid artery balloon injury in rats and the intervention of total glucosides of paeony.
METHODS: The study was approved by the Laboratory Animal Ethical Committee of Shenzhen People’s Hospital. Thirty-six Sprague-Dawley rats were randomly divided into four groups: control group, balloon injury group, total glucosides of paeony group and total glucosides of paeony immersion group. The rats in the control group underwent sham surgery at 4 days after given normal saline via gavage, followed by 13 days of normal saline via gavage. The rats in the balloon injury group underwent left common carotid artery injured by ballooning at 4 days after given normal saline via gavage, followed by 13 days of normal saline via gavage. The rats in the total glucosides of paeony group received balloon injury at 4 days after given total glucosides of paeony via gavage, and then received 13 days of total glucosides of paeony via gavage. The rats in the total glucosides of paeony immersion group received balloon injury at 4 days after given normal saline via gavage, then the balloon was immersed in total glucosides of paeony diluent for 4 hours, and then subjected to 13 days of normal saline via gavage. At 14 days after surgery, the left common carotid artery samples were removed for hematoxylin-eosin staining to observe the changes of intima. The vessels were stained with hematoxylin-eosin. The expression levels of Foxp3 and bone morphogenetic protein 7 mRNA in injured vessels were detected by real-time RT-PCR.
RESULTS AND CONCLUSION: (1) The area of intima and intima/tunica media area in the total glucosides of paeony immersion group were significantly less than those in the total glucosides of paeony group (P < 0.05), and significantly more than those in the control group (P < 0.05). (2) The order of the expression levels of Foxp3 and bone morphogenetic protein 7 mRNA was as follows: control group < balloon injury group < total glucosides of paeony group < total glucosides of paeony immersion group (P < 0.05). (3) These results suggest that total glucosides of paeony can regulate the expression of Foxp3 and bone morphogenetic protein 7 mRNA and the balance of inflammatory factors, inhibit the inflammatory reaction of injured vessels and may contribute to alleviating the restenosis of injured vessels. Local application of balloon immersed in total glucosides of paeony achieved better results than gavage in inhibiting the restenosis caused by inflammation.

Key words: total glucosides of paeony, carotid artery balloon injury, inflammatory cytokines, intima, restenosis, bone morphogenetic protein

CLC Number:

Luo Tedan, Jiang Xin, Zhou Chongchong, Huang Baofeng, Li Peng. Expression Foxp3 and bone morphogenetic protein 7 in rat models of carotid artery balloon injury after treated by total glucosides of paeony[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(35): 5633-5637.

Figures/Tables 3

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