Bone marrow mesenchymal stem cells can effectively treat radiation-induced salivary gland injury by regulating Notch expression

doi:10.3969/j.issn.2095-4344.1786

Abstract

Abstract:

BACKGROUND: Cholinergic receptor agonists can promote the secretion of residual salivary cells in patients with radiation-induced salivary gland injury. However, there are serious adverse reactions associated with its long-term use and limited therapeutic effects on severely radioactive salivary gland damage. Bone marrow mesenchymal stem cells are a kind of cells with multipotential differentiation potential and almost unlimited proliferative capacity, which have the potential to treat radiation-induced salivary gland injury.
OBJECTIVE: To explore the mechanism of bone marrow mesenchymal stem cells repairing radiation-induced salivary gland injury.
METHODS: Bone marrow mesenchymal stem cells were extracted from mouse bone marrow, and co-cultured with the second-generation salivary gland acinar cells for 24 hours by a 3D co-culture system. One hundred and fifty C57 mice were randomly divided into normal control group, radiation-induced salivary gland injury group and bone marrow mesenchymal stem cells+radiation-induced salivary gland injury group (stem cell treatment group). A mouse model of radiation-induced salivary gland injury was made by an electron linear accelerator (15 Gy). One week after irradiation, the mice in the stem cell treatment group were subcutaneously injected with 2×10⁹/L bone marrow mesenchymal stem cell suspension at multiple points of the salivary gland, and those in the other two groups were injected with the same amount of saline.
RESULTS AND CONCLUSION: After 24 hours of co-culture, bone marrow mesenchymal stem cells differentiated into salivary gland acinar cells, with a polygon-like shape and expressed α-amylase. Compared with the normal control group, the salivary flow, salivary gland mass, and salivary amylase level were significantly decreased, acinar cell structure was markedly damaged, and the Notch expression level in the salivary gland was decreased in the radiation-induced salivary gland injury group. Compared with the radiation-induced salivary gland injury group, the above indexes in the bone marrow mesenchymal stem cells+radiation-induced salivary gland injury group were significantly restored. These findings indicate that bone marrow mesenchymal stem cell transplantation can effectively treat radiation-induced salivary gland injury, and its effect may be achieved by regulating the Notch expression level in the salivary gland.

Key words: bone marrow mesenchymal stem cells, cell transplantation, salivary gland, radioactive injury, acinar cells, salivary amylase, Notch, stem cells

CLC Number:

Luo-Meng Yanan, Zhao Na, Ji Nannan, Wang Wufeng.

Bone marrow mesenchymal stem cells can effectively treat radiation-induced salivary gland injury by regulating Notch expression

[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(25): 3967-3972.

Figures/Tables 1

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