Application of beta-tricalcium phosphate/alpha-calcium sulfate hemihydrate combined bone grafts in  multi-segment arthrodesis of spine: micro-CT evaluation

doi:10.3969/j.issn.2095-4344.1731

Abstract

Abstract:

BACKGROUND: Because of the limitation of physico-chemical properties, one kind of graft material alone in spinal arthrodesis cannot match the clinical demands, therefor combined bone grafts consisted of more than two kinds of materials are the issue of concern.

OBJECTIVE: To evaluate the new bone formation and the graft materials resorption of β-tricalcium phosphate/α-calcium sulphate hemihydrate combined bone grafts in rabbit model of multi-segments posterolateral arthrodesis of thoracic vertebrae by micro-CT.

METHODS: Thirty-six rabbits (provide by Experimental Animal Center of General Hospital of PLA) were selected, and their superior/inferior articular processes, bilateral vertebral plate and transverse processes of T_4-8 were removed. β-Tricalcium phosphate/α-calcium sulphate hemihydrate combined bone grafts were transplanted into the lateral posterior part of left vertebral plate of T_4-8 (control group), and autologous bone was transplanted into the another side (control group). The thoracic vertebrae were removed at postoperative 4, 8 and 12 weeks for micro-CT scanning. The morphologic changes of fusion masses were observed. The total bone mineral content, material mineral content, and material volume fraction in the experimental group were analyzed. The experimental protocol was approved by the Animal Ethics Committee of General Hospital of PLA, approval number: 201511007.

RESULTS and CONCLUSION: (1) At the end of 4^th week postoperatively, there were a few osteophytes attached to the laminae, transverse processes and zygapophysial joints at both sides of graft areas. In the experimental group, the morphology of bone graft was clear, and there were few newly born bones surrounding the graft. At the end of 8^th week postoperatively, there were large amount of osteophytes in the graft areas, fusion masses had involved in the complete motion segments, and the newly born bones being surrounded the graft in the experimental group. At the end of 12^th week postoperatively, the fusion masses were enlarged, appeared to be columnar, and the fusion masses were fused into the zygapophysial joints. There were abundant newly born bones surrounding the graft materials in the experimental group, and the scaffold degraded. The fusion mass in the experimental group was obviously larger than that in the control group. (2) The bone mineral content, material mineral content, and material volume fraction in the experimental group were decreased with time. (3) In summary, stable fusion effect of combined bone grafts can be achieved in rabbit models of multi-segments posterolateral thoracic arthrodesis, the combined bone grafts degrade with time, and the degrade speed matches with new bone formation.

Key words: β-tricalcium phosphate, α-calcium sulphate hemihydrate, autologous bone, graft materials, combined bone grafts, combined materials, spinal arthrodesis, multi-segments, rabbit, thoracic vertebrae, micro-CT

CLC Number:

Liang Maohua, Mao Keya, Xia Bo, Liu Qiang, Tang Peifu, Wang Jifang. Application of beta-tricalcium phosphate/alpha-calcium sulfate hemihydrate combined bone grafts in multi-segment arthrodesis of spine: micro-CT evaluation[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(18): 2800-2805.

Figures/Tables 5

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