Platelet-rich plasma promotes the healing of tibial fracture in rabbits

doi:10.3969/j.issn.2095-4344.2882

Abstract

Abstract:

BACKGROUND: There are many questions about fracture healing, such as long self-healing cycles and delayed healing. Biological tissue has the potential for self-repair, so is there a way to mobilize the tissue’s self-repair ability for the organism’s self-repair? Platelet-rich plasma technology is one of the effective ways to solve this problem.

OBJECTIVE: To study the effect of autologous platelet-rich plasmaon the healing of tibial fracture in rabbits.

METHODS: Twelve New Zealand white rabbits were randomly divided into experimental group and control group, with six rabbits in each group. All animals were transversely fractured with a pendulum saw at a site of 2 cm distal to the right tibia, followed by fixation with Kirschner wire. In the experimental group, the platelet-rich plasma gel was injected into the fracture site, and in the control group the same amount of sterile saline was injected into the fracture site. Blood samples from rabbit models were taken before and at 2, 4, and 8 weeks after operation. The contents of osteocalcin and bone alkaline phosphatase were measured. Two rats from each group were killed at 2, 4, and 8 weeks after operation. Repair of fractures and volume of callus were observed and calculated, respectively.

RESULTS AND CONCLUSION: The diameter of the callus in the experimental group was significantly larger than that of the control group at each observation time after operation (P < 0.05). Compared with the control group, the volume of the callus was significantly larger in the experimental group at 4 weeks postoperatively (P < 0.05), and still larger but not significantly at 8 weeks postoperatively (P > 0.05). The average width of trabecular bone, area ratio of trabecular bone and amount of blood vessels in the experimental group were better than those in the control group at 4 weeks postoperatively (P < 0.05); however, there was no significant difference between the two groups at 8 weeks postoperatively (P > 0.05). Serum osteocalcin in the two groups reached the highest level at 4 weeks postoperatively, and could keep a high level for a period of time. The peak level of serum osteocalcin in the experimental group was significantly higher than that in the control group (P < 0.05). The serum alkaline phosphatase level in the experimental group was always higher than that in the control group, indicating that platelet-rich plasma always has an effect on alkaline phosphatase level during fracture healing. To conclude, platelet-rich plasma has a certain promoting effect on the healing of tibial fracture, providing new ideas for treating fracture nonunion or delayed healing.

Key words: bone, fracture, platelet-rich plasma, rabbit, animal, internal fixation, callus

CLC Number:

Ren Jun, Zhao Yan, Xiao Bin, Ma Chao, Wang Xinke, Hao Yabin, Cheng Jie. Platelet-rich plasma promotes the healing of tibial fracture in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(35): 5595-5599.

Figures/Tables 5

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