Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (25): 3943-3946.doi: 10.3969/j.issn.2095-4344.2102

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Cytotoxicity assessment of bone marrow mesenchymal stem cells-cuttlebone composite scaffold  

Peng Ya1, Qin Yu1, Yi Hongcheng2, Gu Chunsong2, Li Lili1   

  1. 1Second Department of Osteopathy, Guizhou Orthopedics Hospital, Guiyang 550002, Guizhou Province, China; 2Second Department of Orthopedics, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China

  • Received:2019-08-23 Revised:2019-08-26 Accepted:2019-10-09 Online:2020-09-08 Published:2020-08-21
  • About author:Peng Ya, Master, Attending physician, Second Department of Osteopathy, Guizhou Orthopedics Hospital, Guiyang 550002, Guizhou Province, China
  • Supported by:

    the Social Development and Livelihood Program of Guiyang Science and Technology Bureau in 2013, No. {2013103}40 ; the Nature Science Research Project of Guizhou Provincial Education Department, No. KY920120040 

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Abstract:

BACKGROUND: Repair materials for bone tissue engineering should hold good biocompatibility and degradability. There are various related studies, but the Chinese medicine composite cellular bioscaffolds are little reported.

OBJECTIVE: To detect the in vitro cytotoxicity of rabbit bone marrow mesenchymal stem cells-cuttlebone bioscaffold based on the Biological Evaluation of Medical Device, and assess its cytotoxicity level in order to provide the theoretical support for its clinical application.

METHODS: Bone marrow mesenchymal stem cells-cuttlebone bioscaffold extract was prepared according to an ISO standard — material area: extraction medium volume = 3-6 cm2:1 mL. L-929 cell suspension was prepared, and the cells were then cultured with a density of 1×107/L. There were three groups: positive group (DMEM medium containing phenol), experimental group (material extract), and negative group (DMEM culture medium). The absorbance value of L-929 cells was detected by MTT assay after 24, 48 and 72 hours of culture. The relative proliferation rate of cells was then calculated and the toxicity level was valued in each group.

RESULTS AND CONCLUSION: The absorbance values in the experimental, negative and positive groups were not exactly same at different time points (P=0.000 < 0.01). The absorbance values in the experimental and negative groups were significantly higher than those in the positive group (P < 0.01).The cytotoxicity of bone marrow mesenchymal stem cells-cuttlebone bioscaffold was grade 1. To conclude, the bone marrow mesenchymal stem cells-cuttlebone bioscaffold has no obvious toxic effects, and meets the requirements of biomaterial application.

Key words:

 cuttlebone,  rabbit bone marrow mesenchymal stem cells,  composite scaffold,  cytotoxicity,  composite bioscaffold

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