Distribution of Sca-1+ adult stem cells along the linea alba of mice

doi:10.3969/j.issn.2095-4344.1721

Abstract

Abstract:

BACKGROUND: The interaction between stem cells and meridians and collaterals is still in the stage of theoretical research, and it is urgent to conduct relevant scientific experiments.
OBJECTIVE: To observe the distributions of Sca-1⁺ adult stem cells in the fat tissues of murine linea alba, providing an experimental basis for further studying the interaction of stem cells with meridians and collateral channels.
METHODS: Balb/c mice were selected to observe the morphological characteristics of any Ren-meridian circulation regions along the anterior midline of the subcutaneous chest of the mice through stereomicroscopy and hematoxylin-eosin staining. Linea alba, the abdomen landmark of Ren-meridian, was selected as the objective of our study. Morphologies of tissues from linea alba were observed by stereomicroscope, hematoxylin-eosin staining, oil red O staining and Masson staining. Distribution of Sca-1⁺ adult stem cells from fat tissues in the linea alba was observed by immunohistochemical staining and multiple slices.
RESULTS AND CONCLUSION: (1) In the study, we firstly observed that the fat tissues presented with band-like distribution in Ren-meridian circulation regions along the anterior midline of the subcutaneous chest of mice. (2) We firstly found that murine linea alba was made of fat tissues. (3) We firstly observed that Sca-1⁺ adult stem cells were distributed along the linea alba, the abdomen landmark of Ren-meridian. The present study preliminarily investigated the Sca-1⁺ adult stem cells in the linea alba part of Ren-meridian. This attempt may provide an experimental basis and idea for the exploration of functions of stem cells and Ren-meridian.

Key words: stem cells, adult stem cells, fat tissue, linea alba, Ren-meridian, Sca-1⁺ adult stem cells, National Natural Science Foundation of China

CLC Number:

Hao Yuhui, Liu Zhizhen, Liu Dan, Feng Yujuan, Xie Jun. Distribution of Sca-1⁺ adult stem cells along the linea alba of mice[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(17): 2672-2677.

Figures/Tables 1

2.1 小鼠皮下前正中线任脉循行部位大体观察结果任脉起于小腹部中极穴下面，沿胸腹正中线直上至咽喉，再上颐、循面、入目[12]。参照小鼠经络穴位示意图，从实验动物中随机选15-16周龄Balb/c小鼠，雌鼠1只，雄鼠1只，进行任脉循行部位解剖定位并取材[13]、体视显微镜观察。小鼠胸部取材厚度从浅筋膜、肌层到胸骨膜，前正中线居中，所取组织长约3.5 cm，宽约2.5 cm。大体可见小鼠胸前正中线任脉走行处有白色脂肪组织呈条带状分布，条带状分布的白色脂肪组织与任脉在胸部的经络循行路线一致，见图1A。小鼠腹部前正中线任脉循行部位解剖定位于腹白线[10-11]，取腹白线组织，厚度从皮下腹壁浅筋膜、腹肌到壁腹膜。腹白线居中，所取组织长约4.5 cm，宽约3.5 cm。体视显微镜下观察到腹白线处由条带状白色脂肪组织构成，条带状分布的白色脂肪组织与任脉在腹部的经络循行路线一致，见图1B。 2.2 小鼠皮下前正中线胸部任脉解剖定位处苏木精-伊红染色观察结果取实验小鼠皮下胸部前正中线任脉解剖定位处组织做苏木精-伊红染色，可见在小鼠皮下前正中线胸部组织冠状面切片上有白色脂肪组织呈条带状分布在胸前正中线，见图2。 2.3 小鼠腹白线苏木精-伊红染色结果取小鼠腹白线及周围组织进行苏木精-伊红染色，结果可见在腹白线组织冠状面切片上有白色脂肪组织呈条带状分布，见图3。 2.4 小鼠腹白线组织特殊染色观察结果取小鼠腹白线组织进行油红O染色与Masson染色，观察到在小鼠腹白线组织冠状面切片腹白线上有油红O染色阳性的脂肪细胞与Masson染色阳性的胶原纤维，见图4；而分布在腹白线两边的是染成红色的肌纤维。组织特殊染色结果显示小鼠腹白线由白色脂肪组织构成。 2.5 Sca-1+成体干细胞在小鼠腹白线脂肪组织中的分布情况腹白线脂肪组织多层切片中均有染成棕色的Sca-1+细胞，而与其相邻的腹前壁肌层中未见染成棕色的Sca-1+细胞，见图5(箭头所示)。"

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