Preparation and properties of recombinant collagen sponge

doi:10.3969/j.issn.2095-4344.1580

Abstract

Abstract:

BACKGROUND: Recombinant collagen has good hydrophilicity and biocompatibility, but it has the disadvantages of strong solubility and low mechanical strength. Chemical crosslinking can significantly improve the toughness, mechanical strength and degradation resistance of the material.

OBJECTIVE: To prepare recombinant collagen sponge and to evaluate its physicochemical properties and safety.

METHODS: The recombinant collagen was obtained via microbial fermentation and cross-linked with glutaraldehyde. The porous recombinant collagen sponge was finally obtained by freeze-drying in the mold. The water absorption, porosity and crosslinking agent residual of the recombinant collagen sponge were detected. The surface morphology of the recombinant collagen sponge before and after cross-linking was observed by scanning electron microscope. The changes in recombinant collagen structures before and after cross-linking were compared by infrared spectrometer. L929 cells were cultured with cross-linked recombinant collagen sponge extract. At 68 hours after culture, the relative proliferation rate of the cells was detected by MTT, and the cytotoxicity of cross-linked recombinant collagen sponge was evaluated.

RESULTS AND CONCLUSION: (1) The water absorption of the cross-linked collagen sponge was (2 903.83±47.90)% and the average porosity was over 85%. (2) Under the scanning electron microscope, the pores of the collagen sponge were honeycomb and dense before cross-linking. After cross-linking, the pores of the collagen sponge were large and bridged bond linkage of laminar layer structure could be seen, and also the large folds could be seen in the longitudinal direction. (3) There was no obvious change in the characteristic chemical structure of the recombinant collagen sponge after cross-linking. (4) The relative proliferation rate of L929 cells was 84% after culture in cross-linked recombinant collagen sponge extract, and the cytotoxicity was level 1, indicating the biological safety was good. To conclude, the recombinant collagen sponge with glutaraldehyde cross-linking shows stable physicochemical properties and good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen, Hemostasis, Cross-Linking Reagents;, Tissue Engineering

CLC Number:

R459.9

R318.08

He Yue, Hou Zengmiao, Li Xiaoying, Gao En, Liu Jianli, Zhao Jinli. Preparation and properties of recombinant collagen sponge[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(6): 912-916.

Figures/Tables 5

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