Osteogenic property of beta-tricalcium phosphate/alpha-calcium sulphate hemihydrate combined bone graft in multi-segment posterolateral arthrodesis of rabbit thoracic vertebrae

doi:10.3969/j.issn.2095-4344.1277

Abstract

Abstract:

BACKGROUND: Preliminary studies have revealed that β-tricalcium phosphate/α-calcium sulphate hemihydrate combined bone graft has ideal biomechanical strength and degradation performance in vitro.

OBJECTIVE: To observe the new bone formation and resorption of β-tricalcium phosphate/α-calcium sulphate hemihydrate combined bone graft in rabbit models of multi-segment posterolateral arthrodesis of thoracic vertebrae.

METHODS: Thirty-six male New Zealand white rabbits (provided by Experimental Animal Center of General Hospital of PLA) were selected and their superior/inferior articular processes, bilateral vertebral plate and transverse processes of T₄-T₈ were removed. β-Tricalcium phosphate/α-calcium sulphate hemihydrate combined bone grafts were transplanted into the right side (experimental group), and autologous bone was transplanted into the another side (control group). Tetracycline hydrochloride was injected through vein as fluorescent mark 13 and 3 days before the rabbits were euthanized. Thoracic segments of spine were removed at 4, 8 and 12 weeks postoperatively. Tetracycline hydrochloride staining was used to observe the morphology of newly formed bones. Molybdenum soft X-ray was used for morphological observation and histological observation was conducted. The experimental protocol was approved by the Animal Ethics Committee of General Hospital of PLA, approval number: 201511007.

RESULTS AND CONCLUSION: (1) The new bone mineral appositional rate of graft materials in the two groups decreased with time, and peaked at the end of postoperative 4 weeks. The new bone mineral appositional rate in the graft area at postoperative different time points in the experimental group was lower than that in the control group (P > 0.05). (2) Mammography revealed that the contours of combined bone grafts and boundaries between graft and bones were clear at the end of postoperative 4 weeks, but contours of combined bone grafts were blurred at the end of postoperative 8 weeks and the new bones grew into scaffolds of combined bone grafts. The graft materials were crawling substituted by new bones at the end of postoperative 12 weeks, and there were only small amount of remains at the central of graft sites. (3) Giemsa staining showed that combined bone graft materials degraded gradually, and were surrounded by new bone over time; these materials had completely degraded into fine fragments by the end of 12 weeks after operation. (4) Our results indicate that β-tricalcium phosphate/α-calcium sulphate hemihydrate combined graft in the multi-segment posterolateral arthrodesis of rabbit thoracic vertebrae holds good osteogenic property and degradation property.

Key words: β-tricalcium phosphate, α-calcium sulphate hemihydrate, combined bone graft, bone graft, biomaterial, combined material, spinal arthrodesis, multi-segments, rabbit, thoracic vertebrae, posterolateral approach

CLC Number:

Liang Maohua, Mao Keya, Xia Bo, Liu Qiang,Tang Peifu, Wang Jifang. Osteogenic property of beta-tricalcium phosphate/alpha-calcium sulphate hemihydrate combined bone graft in multi-segment posterolateral arthrodesis of rabbit thoracic vertebrae[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(22): 3500-3505.

Figures/Tables 6

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