Inhibitory effects of nanosilver sponge with Danhuang powder on Pseudomonas aeruginosa

doi:10.3969/j.issn.2095-4344.1276

Abstract

Abstract:

BACKGROUND: Danhuang powder has been recently shown to promote the healing of diabetic foot ulcers, but its antibacterial activity is not satisfactory. Therefore, broad-spectrum antibacterial nanosilver sponge is loaded with Danhuang powder for clinical application. OBJECTIVE: To investigate the antibacterial activity of nanosilver sponge with Danhuang powder against Pseudomonas aeruginosa.

METHODS: Nanosilver sponge with Danhuang powder containing 22.22%, 16.67%, 11.11% silver nitrate (Danhuang-nanosilver group) and simple nanosilver sponge containing 22.22%, 16.67%, 11.11% silver nitrate (simple nanosilver group) were cut into round pieces (1 cm in diameter) and placed on Pseudomonas aeruginosa suspension-spread culture medium. Paper sheets of the same size were dipped in the imipenem-cilastatin aqueous solution (imipenem-cilastatin group) and then also positioned on Pseudomonas aeruginosa suspension-spread culture medium for positive controls. For negative controls, chitosan sponge of the same size was used (chitosan group). All tested materials were cultured in an incubator at 35 °C for 24 hours, and the size of the inhibition zone was compared.

RESULTS AND CONCLUSION: The antibacterial activity against Pseudomonas aeruginosa in the imipenem-cilastatin group was superior to that in the Danhuang-nanosilver, simple nanosilver, chitosan groups (P < 0.05). The antibacterial activity against Pseudomonas aeruginosa in the Danhuang-nanosilver and simple nanosilver groups was superior to those in the chitosan group (P < 0.05). The antibacterial activity against Pseudomonas aeruginosa was similar between Danhuang-nanosilver and simple nanosilver groups at the same concentration of silver nitrate (P > 0.05). There was no significant difference in the antibacterial activity against Pseudomonas aeruginosa between different concentrations of silver nitrate in the simple nanosilver and Danhuang-nanosilver groups (both P > 0.05). These results suggest that nanosilver sponge with Danhuang powder exhibits antibacterial activity against Pseudomonas aeruginosa, showing unique advantages in the treatment of diabetic foot ulcers.

Key words: nanosilver sponge with Danhuang powder, Danhuang-nanosilver sponge, nanosilver sponge, imipenem-cilastatin, chitosan sponge, nanosilver, Pseudomonas aeruginosa, antibacterial effects, diabetic foot ulcers

CLC Number:

R459.9','1');return false;" target="_blank">
R459.9

R318.08

Cite this article

Li Tao, Zhang Chunling, Long Yi, Di Tietao, Chen Lu, Zhao Wei, Long Li, Huang Qiang, Tang Lisha, Luo Kaizhong. Inhibitory effects of nanosilver sponge with Danhuang powder on Pseudomonas aeruginosa [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(22): 3495-3499.

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URL: https://www.cjter.com/EN/10.3969/j.issn.2095-4344.1276

https://www.cjter.com/EN/Y2019/V23/I22/3495

Figures/Tables 5

2.1 各组抑菌实验结果采用IMAGEJ测算各组抑菌面积，为确保结果的精确度，将亚胺培南-西司他丁组抑菌面积作为基数值，计算出各干预组面积比值，见表2。"

2.2 各组之间抑菌效果的比较亚胺培南-西司他丁组的抑菌效果优于纳米银海绵组，见图1。亚胺培南-西司他丁组的抑菌效果优于各浓度丹银海绵组(P < 0.05)，见图2。亚胺培南-西司他丁组的抑菌效果优于壳聚糖海绵组 (P < 0.05)，见图3。各浓度纳米银海绵组的抑菌效果优于壳聚糖海绵组(P < 0.05)，见图4。各浓度丹银海绵组的抑菌效果优于壳聚糖海绵组(P < 0.05)，见图5。相同硝酸银浓度下，纳米银海绵组和丹银海绵组具有同等的抑菌效果(P > 0.05)，见图6。不同浓度的纳米银海绵抑菌效果无差异(P > 0.05)，见图7。不同浓度的丹银海绵抑菌效果无差异(P > 0.05)，见图8。"

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