Changes of Toll-like receptor 4/myeloid differentiation factor 88 signal transduction in rat models of knee osteoarthritis treated with massage

doi:10.3969/j.issn.2095-4344.1188

Abstract

Abstract:

BACKGROUND: Toll-like receptor 4/myeloid differentiation factor 88 signal transduction pathway has been shown to induce various inflammatory reactions, and the occurrence of knee osteoarthritis is closely related to Toll-like receptor 4/myeloid differentiation factor 88 signal transduction pathway. Massage can reduce inflammatory factors such as interleukin 6 and tumor necrosis factor alpha to exert anti-inflammatory and analgesic role, and has a certain effect on the prevention and treatment of knee osteoarthritis.
OBJECTIVE: To explore the expression of Toll-like receptor 4, myeloid differentiation factor 88, tumor necrosis factor receptor associated factor 6 and nuclear factor kappa B p65 mRNA and protein in a rat model of knee osteoarthritis treated with massage, and to explore the effect and mechanism of massage in the treatment of knee osteoarthritis.
METHODS: Ten rats were randomized into blank group and the remaining 40 rats were injected with papain to establish knee osteoarthritis model. The rat models were randomly divided into model, massage, celecoxib and massage plus celecoxib groups (n=10/group). The blank and model groups were fed routinely. The massage group was treated with massage using one finger. The celecoxib group was given intragastric administration of celecoxib. The massage plus celecoxib group was given the above two treatments. Each group was treated for 4 weeks.
RESULTS AND CONCLUSION: (1) Compared with the model group, massage and/or celecoxib treatment could significantly reduce the Mankin score of cartilage in rats. (2) Compared with the model group, massage and/or celecoxib treatment could significantly reduce the expression levels of Toll-like receptor 4, myeloid differentiation factor 88, nuclear factor kappa B p65 and tumor necrosis factor receptor associated factor 6 mRNA and protein in cartilage tissue of rats. The expression levels of above cytokines in the massage and celecoxib group were similar to the blank group. (3) These results suggest that massage prevents and treats knee osteoarthritis probably through down-regulating the expression of Toll-like receptor 4, myeloid differentiation factor 88, tumor necrosis factor receptor associated factor 6, nuclear factor kappa B p65 mRNA and protein, thus delaying the progression of knee osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: knee osteoarthritis, celecoxib, massage, Toll-like receptor 4, myeloid differentiation factor 88, nuclear factor kappa B p65, tumor necrosis factor 6, cartilage

CLC Number:

Liu Jiao, , , Qu Chongzheng, , Xie Pingjin, , , Ye Dalin, , , Luo Zhen, , , Liang Zhenghui, , , Tao Jing, , Jiang Ganghui, . Changes of Toll-like receptor 4/myeloid differentiation factor 88 signal transduction in rat models of knee osteoarthritis treated with massage[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(19): 3019-3024.

Figures/Tables 2

2.1 实验动物数量分析实验过程中无大鼠死亡，除为验证造模是否成功每组处死1只大鼠外，其余均完成所有干预治疗，进入结果分析。 2.2 推拿对膝骨性关节炎大鼠软骨大体形态及组织学形态的影响除空白组外，各组均出现不同程度的软骨损伤，主要损失部位为股骨内踝及内侧胫骨平台。如图2所示，推拿组与推拿加塞来昔布组均可见软骨表层欠平整，无裂隙，少数软骨细胞出现成簇现象，基质染色基本无异常；塞来昔布组的染色颜色较轻，软骨表面有裂隙；模型组软骨表面粗糙，组织结构层次紊乱，软骨细胞增多，较多成簇的软骨细胞。如图3示，与空白组相比，其余各组改良版Mankin评分较高(P < 0.05)。与模型组相比，推拿加塞来昔布组、推拿组以及塞来昔布组改良Mankin评分较低(P < 0.05)，且改良Mankin评分推拿加塞来昔布组<推拿组<塞来昔布组，差异有显著性意义(P < 0.05)。 2.3 推拿对膝骨性关节炎大鼠软骨中TLR4 、MYD88、NF-κΒp65及TRAF6 mRNA表达水平的影响与空白组相比，模型组、推拿组以及塞来昔布组的TLR4，MYD88，NF-κΒp65以及TRAF6 mRNA表达水平均上调(P < 0.05)，推拿+塞来昔布组与空白组TLR4，MYD88和NF-κΒp65 mRNA表达水平接近(P > 0.05)；与模型组相比，推拿加塞来昔布组、推拿组以及塞来昔布组TLR4，MYD88，NF-κΒp65以及TRAF6 mRNA表达水平下调(P < 0.05)，且TLR4，MYD88，NF-κΒp65以及TRAF6 mRNA表达水平：推拿+塞来昔布组<推拿组<塞来昔布组(P < 0.05)，见图4。2.4 推拿对膝骨性关节炎大鼠软骨TLR4，MYD88，NF-κΒp65及TRAF6蛋白表达水平的影响与空白组相比，其余各组TLR4，MYD88，NF-κΒp65及TRAF6蛋白的表达水平上调(P < 0.05)；与模型组相比，推拿+塞来昔布组、推拿组以及塞来昔布组TLR4，MYD88，NF-κΒp65及TRAF6蛋白的表达水平下调(P < 0.05)；且TLR4，MYD88，NF-κΒp65及TRAF6蛋白表达水平：推拿加塞来昔布组<推拿组<塞来昔布组(P < 0.05)，见图5，表3。"

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