Effect of glucosamine capsule on cartilage metabolism-related genes in peripheral blood mononuclear cells of patients with knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2108

Abstract

Abstract:

BACKGROUND: At present, the research on glucosamine mostly focused on the treatment of knee osteoarthritis, but the research on the effect of glucosamine on cartilage metabolism-related genes in peripheral blood of patients with knee osteoarthritis is limited.

OBJECTIVE: To observe the effect of glucosamine capsule on knee osteoarthritis and the expressions of cartilage metabolism-related genes.

METHODS: Totally 90 knee osteoarthritis patients admitted to the Zhengzhou Central Hospital Affiliated to Zhengzhou University from March 2017 to February 2019 were selected, and 40 healthy subjects from the medical examination center in the same period were selected as healthy subjects. The cartilage oligomeric matrix protein (COMP), type II collagen fiber α1 gene (COL2A1), glycoprotein and specific tissue inhibitor (TIMP)-3 gene expression levels in peripheral blood mononuclear cells were detected and compared between healthy subjects and knee osteoarthritis patients before treatment. Knee osteoarthritis patients were divided into routine group and study group by random number table, and they were treated with diclofenac sodium sustained-release tablets, diclofenac sodium sustained-release tablets and glucosamine capsules, respectively. Both groups were treated for 12 weeks. The levels of gene expressions and Lequesne index in peripheral blood mononuclear cells of two groups were compared before and after treatment. During the treatment, the occurrence of adverse reactions was counted in the two groups.

RESULTS AND CONCLUSION: (1) The relative expressions of COMP and TIMP-3 in peripheral blood mononuclear cells of knee osteoarthritis patients were higher than those of healthy subjects (P < 0.05), while the mRNA relative expressions of COL2A1 and glycoprotein were lower than those of healthy subjects (P < 0.05). (2) There were no significant difference in gene expression levels in peripheral blood mononuclear cells before treatment between study group and routine group (P > 0.05). The mRNA relative expressions of COMP and TIMP-3 in peripheral blood mononuclear cells of the two groups after treatment were lower than those before treatment, and those of the study group were lower than those of the routine group (P < 0.05). The mRNA relative expressions of COL2A1 and glycoprotein in peripheral blood mononuclear cells of the two groups after treatment were higher than those before treatment, and the mRNA relative expressions of COL2A1 and ACAN in the study group were higher than those in the routine group (P < 0.05). (3) There was no significant difference in Lequesne index between the study group and the routine group before treatment (P > 0.05). Lequesne index in both groups after treatment was lower than that before treatment, and that in the study group was lower than that in the routine group (P < 0.05). (4) There was no significant difference in the incidence of adverse reactions between the study group and the routine group (P > 0.05). (5) Results suggest that glucosamine capsule can effectively improve the clinical symptoms of patients with knee osteoarthritis. It is safe and reliable. It may promote the expression of COL2A1 and glycoprotein genes by inhibiting the expression of COMP and TIMP-3 genes.

Key words: glucosamine capsule, knee osteoarthritis, peripheral blood mononuclear cells, cartilage oligomeric matrix protein, type II collagen fiber α1 gene, glycoprotein, specific tissue inhibitor-3

CLC Number:

Xiao Lei, Hou Ligang. Effect of glucosamine capsule on cartilage metabolism-related genes in peripheral blood mononuclear cells of patients with knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 5007-5012.

Figures/Tables 7

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