Overexpression of heme oxygenase-1 promotes biosynthesis of skeletal muscle mitochondria during hypoxia training in mice

doi:10.3969/j.issn.2095-4344.1181

Abstract

Abstract:

BACKGROUND: Studies on hypoxic training concentrate on the regulatory role of hypoxic training in blood system, but the results have dual characteristics, revealing its regulatory role in non-blood system.
OBJECTIVE: To investigate the regulation of heme oxygenase-1 in mitochondria and biosynthesis in mouse skeletal muscle during hypoxic training.
METHODS: Twenty male 8-week-old overexpression heme oxygenase-1 transgenic mice and 20 wild-type mice, SPF grade were provided by School of Medicine of Lanzhou University. The two species were randomly divided into four groups: wild-type mice hypoxia control, wild-type mice hypoxia training, transgenic mice control and transgenic mice hypoxia training groups. The mice in the hypoxia training groups underwent treadmill running (20 m/min, slope 5°, 60 min/d, 5 d/week, for 4 weeks) at Basic Medical Research Center of Qinghai University. The treadmill running was undergone in each group at 3 days after final training, and the exhaustive exercise time was recorded. The number of mitochondria in skeletal muscle was detected by electron microscope. Mitochondrial membrane potential was determined using JC-1 fluorescent probe. ATP synthesis capacity was determined using a bioluminescence technique. The protein expression of heme oxygenase-1, cytochrome C oxidase IV and peroxisome proliferator-activated receptor gamma cofactor 1 alpha was detected by western blot assay.
RESULTS AND CONCLUSION: (1) Compared with the wild-type mice hypoxia control group, the exhausted exercise time extended in the wild-type mice hypoxia training and transgenic mice hypoxia training groups, the number of mitochondria was slightly increased, the membrane potential and ATP synthesis capacity were elevated, and protein expression levels of heme oxygenase-1, cytochrome C oxidase IV and peroxisome proliferator-activated receptor gamma cofactor 1 alpha were significantly increased. (2) Compared with the wild-type mice hypoxia training group, the exhausted exercise time in the transgenic mice hypoxia training group was significantly increased, the number of mitochondria was significantly increased, the membrane potential and ATP synthesis capacity were significantly elevated, and protein expression levels of heme oxygenase-1, cytochrome C oxidase IV and peroxisome proliferator-activated receptor gamma cofactor 1 alpha were significantly increased. (3) These results indicate that heme oxygenase-1 has the effect of prolonging exhaustion time in hypoxic training. The mechanism may be related to the promotion of mitochondrial number and biosynthesis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: heme oxygenase-1, hypoxia training, skeletal muscle, mitochondria, membrane potential, exhaustive exercise, ATP synthesis, mitochondria membrane potential, the National Natural Science Foundation of China

CLC Number:

R446
R329.21

Zhao Yanli, Qin Hailan, Cui Hao, Gan Luoman, Si Lining. Overexpression of heme oxygenase-1 promotes biosynthesis of skeletal muscle mitochondria during hypoxia training in mice[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(19): 2991-2995.

Figures/Tables 5

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