Effect of combined application of extracorporeal circulation perfusion system and Mailuoning on crush injury and crush syndrome in pig models

doi:10.3969/j.issn.2095-4344.1141

Abstract

Abstract:

BACKGROUND: Severe crush injury and crush syndrome have high disability and mortality rates, which seriously threaten the lives of the injured. Reducing the return of harmful substances into blood after extrusion relief is the key to prevent and treat crush syndrome.
OBJECTIVE: To investigate the effect of combined application of extracorporeal circulation perfusion system and Mailuoning injection on crush injury and crush syndrome of lower extremity in pigs.
METHODS: Twenty-four Bama miniature pigs (provided by Beijing Tonghe Shengtai Institute for Comparative Medicine) were randomly divided into four groups (n=6/group): blank control, modeling, routine perfusion, and Mailuoning perfusion groups. The pigs in the latter three groups were used to establish the crush injury-crush syndrome model by self-made crush device. The modeling group restored blood supply after modeling immediately. The routine perfusion and Mailuoning perfusion groups respectively restored after receiving routine blood perfusion and Mailuoning perfusion treatment for 1 hour.
RESULTS AND CONCLUSION: The survival rate at 48 hours in the routine perfusion and Mailuoning perfusion groups were higher than that in the modeling group. Levels of creatinine, blood urine nitrogen, potassium ion concentration, myoglobin, creatine kinase, interleukin-1 beta and interleukin-6 in in the routine perfusion and Mailuoning perfusion groups were significantly decreased (P < 0.05). The levels of myoglobin and creatine kinase in the Mailuoning perfusion group at 6 hours were significantly lower than those in the conventional perfusion group (P < 0.05). Compared with the modeling and conventional perfusion groups, the malonaldehyde level in skeletal muscle, and contents of cytochrome C and apoptosis inducing factor in cytoplasm in the cytoplasm Mailuoning perfusion group were significantly decreased (P < 0.05), and the superoxide dismutase activity, and the contents of cytochrome C and apoptosis inducing factor in mitochondria were significantly increased (P < 0.05). Transmission electron microscope showed that the ultrastructure of skeletal muscle in the Mailuoning perfusion group was closer to the normal tissue than the modeling and conventional perfusion groups. The tissue injury condition was similar between modeling and conventional perfusion groups. These results indicate that the combined application of the extracorporeal circulation perfusion system and Mailuoning can prevent and treat the crush injury crush syndrome of the pig models and provide a new idea for the treatment of this disease.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Extracorporeal Circulation, Crush Syndrome, Tissue Engineering

CLC Number:

R453
R364

Wang Lei1, Nie Xin1, Yin Yefeng2, Qin Xinyuan2, Chen Tiangui2, Gao Lei2, Lu Lizhen1, Wang Jiangning2 . Effect of combined application of extracorporeal circulation perfusion system and Mailuoning on crush injury and crush syndrome in pig models[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(11): 1723-1729.

Figures/Tables 4

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