Role of mechano growth factor in the proliferation and migration of rabbit knee articular chondrocytes

doi:10.3969/j.issn.2095-4344.1108

Abstract

Abstract:

BACKGROUND: Mechano growth factor exists in a wide variety of tissues, and it can enhance the proliferation and migration of cells in different tissues.
OBJECTIVE: To investigate the effect of mechano growth factor on the proliferation and migration of primary rabbit knee articular chondrocytes, and to explore the underlying mechanism.
METHODS: Knee cartilage was shredded after being harvested from New Zealand white rabbits (provided by Beijing Vital River Laboratory Animal Technology Co., Ltd.), and then chondrocytes were isolated and cultured by enzyme digestion. Passage 3 cells in logarithmic growth phase were harvested and divided into five groups, and then intervened by 0, 1, 2, 4 and 8 μg/L mechano growth factor for 24, 48 and 72 hours, respectively.
RESULTS AND CONCLUSION: Mechano growth factor could promote the proliferation and migration of rabbit knee articular chondrocytes in a concentration-dependent manner, and mechano growth factor at 4 μg/L exhibited the strongest efficacy. Mechano growth factor could increase the expression of transforming growth factor β1 and phosphorylation of Smad2/3 with concentration increasing. In summary, mechano growth factor can promote the proliferation and migration of rabbit knee articular chondrocytes dose-dependently, which may be via the transforming growth factor β1/Smad signaling pathway.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Cytokines, Smad Proteins, Transforming Growth Factors, Tissue Engineering

CLC Number:

R456
R363

Zhao Hui, Zhou Junlin, Yang Tiejun, Li Yihan, Guo Meng . Role of mechano growth factor in the proliferation and migration of rabbit knee articular chondrocytes [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(11): 1674-1679.

Figures/Tables 1

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