Ultrastructural evaluation of a rabbit model of steroid-induced osteonecrosis of the femoral head after treatment with panax notoginseng saponins

doi:10.3969/j.issn.2095-4344.1072

Abstract

Abstract:

BACKGROUND: Preliminary studies have shown that panax notoginseng saponins can promote the repair of osteocyte ultrastructure in a rabbit with alcohol-induced osteonecrosis of the femoral head.
OBJECTIVE: To observe the structural changes of osteocytes in a rabbit with steroid-induced osteonecrosis of the femoral head treated with panax notoginseng saponins by transmission electron microscope.
METHODS: Ninety 9-10 weeks old healthy male New Zealand rabbits (provided by Laboratory Center of Guangxi University of Chinese Medicine), weighting 3.5-4.0 kg were selected. Rabbit models of steroid-induced osteonecrosis of the femoral head were established by injecting modified horse serum combined with methylprednisolone sodium succinate. Fifteen rabbits died, so the remaining 75 rabbits were divided into three groups (n=25 per group), and then treated with normal saline, cervus and cucumis polypeptide or panax notoginseng saponins, respectively for 5 weeks. The structural changes of osteocytes were observed under transmission electron microscope.
RESULTS AND CONCLUSION: In the normal saline group, the number of mature osteocytes was reduced, vascular endothelial cells were damaged, and mitochondria swelling, fuzzy cristae structure, nuclear fragmentation, and lipid droplets were observed. In the cervus and cucumis polypeptide and panax notoginseng saponins groups, there were more mature osteocytes and less vascular endothelial cell damage, swollen mitochondria alleviated with clear structure, and the numbers of mast adocytes and lipid droplets were decreased. The structural changes of osteocytes in the cervus and cucumis polypeptide and panax notoginseng saponins groups were significantly more encouraging than those in the normal saline group (P < 0.05). Our results indicate that show that, panax notoginseng saponins can repair the osteocyte structure of rabbits with steroid-induced osteonecrosis of the femoral head.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Ginsenosides, Femur Head Necrosis, Models, Animal, Microscopy, Electron, Transmission, Tissue Engineering

CLC Number:

R446

Han Jie1, Chen Yueping1, Mo Jian1, Wang Dawei1, Su Bo1, Li Shuzhen1, Xia Tian2, Wang Shixin2. Ultrastructural evaluation of a rabbit model of steroid-induced osteonecrosis of the femoral head after treatment with panax notoginseng saponins [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1035-1039.

Figures/Tables 2

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