A chemical radiculitis model in the rat: establishment and evaluation

doi:10.3969/j.issn.2095-4344.1065

Abstract

Abstract:

BACKGROUND: Chemical nerve root modeling experiments provide a stable animal model for studying the intervertebral discs.
OBJECTIVE: To explore the establishment of lumbar disc herniation and chemical nerve root inflammation in rats by formaldehyde soaked filter paper, and to find the best operation plan and the solution concentration.
METHODS: Ten Sprague-Dawley rats provided by Beijing Huafukang Bioscience Co., Inc., in China were randomly divided into two groups and subjected to biting off the spinous process or not. Then, 4% formaldehyde soaked filter papers were placed beside the dorsal root nerve to make the model. Based on behavioral performance, the optimal surgical plan was determined. Thereafter, the effect of different concentrations of formaldehyde soaked filter papers on the modeling. Another 20 Sprague-Dawley rats were randomized into 4 groups, 15 of which were subjected to the above-determined surgical plan of biting off the spinous process, and 1%, 2% and 4% formaldehyde soaked filter papers were placed beside the dorsal root nerve. Siegal neurological score, hematoxylin-eosin staining, and measurement of related inflammatory factors were used to assess modeling effects.
RESULTS AND CONCLUSION: The Siegal neurological score and grading were significantly increased in the rats with biting off the L4, 5 than those without biting off the spinous process. Compared with the control group, the Siegal neurological score and grading were markedly increased in 1%, 2%, 4% formaldehyde groups at 2-8 days after modeling, but this increasing tendency remained only in the 2% and 4% formaldehyde groups within 2 weeks after modeling. Moreover, significant inflammatory cell infiltrations occurred in the rat’s nerve, muscle and intervertebral disc, and the levels of serum related inflammatory factors increased remarkably. These findings indicate that the improved modeling method has high successful rate and low mortality, which can provide ideal animal models for subsequent diseases and drug researches.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Formaldehyde, Radiculopathy, Tissue Engineering

CLC Number:

R441
R361

Zhao Caiping, Zhu Meiling, Wang Tingting, Liu Xuelei, Liu Cuiling. A chemical radiculitis model in the rat: establishment and evaluation[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1030-1034.

Figures/Tables 4

References

[1] Clarke LE, Richardson SM, Hoyland JA. Harnessing the Potential of Mesenchymal Stem Cells for IVD Regeneration. Curr Stem Cell Res Ther. 2015;10(4):296-306.

[2] 李雷.不同手术方式对腰椎间盘突出症患者术后生活质量影响[D].泰安:泰山医学院,2015.

[3] Bae WC, Masuda K. Emerging technologies for molecular therapy for intervertebral disk degeneration. Orthop Clin North Am. 2011; 42(4):585-601, ix.

[4] Daly C, Ghosh P, Jenkin G, et al. A Review of Animal Models of Intervertebral Disc Degeneration: Pathophysiology, Regeneration, and Translation to the Clinic. Biomed Res Int. 2016;2016: 5952165.

[5] Sakai D, Nishimura K, Tanaka M, et al. Migration of bone marrow-derived cells for endogenous repair in a new tail-looping disc degeneration model in the mouse: a pilot study. Spine J. 2015;15(6):1356-1365.

[6] 高元朝,赵志斌,支满霞,等.脉络宁注射液与激素对脊神经根炎所致脊髓损伤保护作用的比较[J].中国实验方剂学杂志, 2014;20(21): 187-192.

[7] 刘洋,王东风,黄娜.复方南星蠲瘀软膏对大鼠神经根炎和足趾肿胀的药理作用研究[J].中国药房,2011,22(11):977-979.

[8] 王林林,李明,金菲.电针疗法对神经根型颈椎病模型大鼠神经根保护作用机理研究[J].辽宁中医药大学学报, 2014,16(6):111-114.

[9] 舒小琴.SD大鼠断层解剖学彩色图谱的构建[D].武汉:华中科技大学, 2011.

[10] 唐朝辉,曹亮,丁岗,等.腰痹通胶囊对大鼠腰椎神经根压迫致腰椎间盘突出模型药效及炎性因子的影响[J].中国实验方剂学杂志, 2015, 21(5):155-158.

[11] Hoy D, March L, Brooks P, et al. The global burden of low back pain: estimates from the Global Burden of Disease 2010 study. Ann Rheum Dis. 2014;73(6):968-974.

[12] 陈柏书.针刺治疗腰椎间盘突出症的去自由基镇痛机理研究[D].广州:广州中医药大学,2014.

[13] 王美琴,郭长青,张义,等.针刀疗法对腰椎间盘突出症大鼠背根神经节局部微循环影响的实验研究[J].针灸临床杂志, 2016,32(1):72-75.

[14] 刘进显,黎尚荣.大鼠腰神经根受压模型建立的实验研究[J].现代医院, 2010,10(5):23-24.

[15] Cuéllar JM, Borges PM, Cuéllar VG, et al. Cytokine expression in the epidural space: a model of noncompressive disc herniation- induced inflammation. Spine (Phila Pa 1976). 2013;38(1):17-23.

[16] Zhu X, Cao S, Zhu MD, et al. Contribution of chemokine CCL2/CCR2 signaling in the dorsal root ganglion and spinal cord to the maintenance of neuropathic pain in a rat model of lumbar disc herniation. J Pain. 2014;15(5):516-526.

[17] Vassilaki M, Hurwitz EL. Insights in public health: perspectives on pain in the low back and neck: global burden, epidemiology, and management. Hawaii J Med Public Health. 2014;73(4):122-126.

[18] Yuan W, Che W, Jiang YQ, et al. Establishment of intervertebral disc degeneration model induced by ischemic sub-endplate in rat tail. Spine J. 2015;15(5):1050-1059.

[19] Fujioka Y, Stahlberg A, Ochi M, et al. Expression of inflammation/ pain-related genes in the dorsal root ganglion following disc puncture in rats. J Orthop Surg (Hong Kong). 2016;24(1):106-112.

[20] Numaguchi S, Esumi M, Sakamoto M, et al. Passive cigarette smoking changes the circadian rhythm of clock genes in rat intervertebral discs. J Orthop Res. 2016;34(1):39-47.

[21] 赵赫,俞兴,唐向盛,等.炎性因子IL-1β?TNF-α与椎间盘退变关系的研究进展[J].中国骨伤,2017,30(9):866-871.

[22] Chung JM, Kim HK, Chung K. Segmental spinal nerve ligation model of neuropathic pain. Methods Mol Med. 2004;99:35-45.

[23] Zamora T, Palma J, Andia M, et al. Effect of Propionibacterium acnes (PA) injection on intervertebral disc degeneration in a rat model: Does it mimic modic changes? Orthop Traumatol Surg Res. 2017;103(5):795-799.

[24] Tong W, Lu Z, Qin L, et al. Cell therapy for the degenerating intervertebral disc. Transl Res. 2017;181:49-58.

[25] Song H, Luo Y, Wang W, et al. Effects of alendronate on lumbar intervertebral disc degeneration with bone loss in ovariectomized rats. Spine J. 2017;17(7):995-1003.

[26] Luo Y, Zhang L, Wang WY, et al. Alendronate retards the progression of lumbar intervertebral disc degeneration in ovariectomized rats. Bone. 2013;55(2):439-448.

[27] Jia H, Ma J, Lv J, et al. Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/β-catenin pathway activity. Sci Rep. 2016;6:27521.

[28] 徐海栋,刘晓伟,史新瑞,等.大鼠尾椎椎间盘退变静态压力模型的构建[J].脊柱外科杂志,2017,15(6):348-350,356.

[29] Nakayama E, Matsumoto T, Kazama T, et al. Transplantation of dedifferentiation fat cells promotes intervertebral disc regeneration in a rat intervertebral disc degeneration model. Biochem Biophys Res Commun. 2017;493(2):1004-1009.

[30] 苏清,赵瑞青,任伟云.腹膜透析中预防腹腔感染的护理对策[J].中原医刊,2003,30(21):57.