Spinal cord stimulation alters the serum levels of inflammatory factors in canine atrial fibrillation models

doi:10.3969/j.issn.2095-4344.1026

Abstract

Abstract:

Abstract
BACKGROUND: Some studies have shown that spinal cord stimulation can inhibit atrial fibrillation by regulating the balance of cardiac autonomic nerves. However, it is less reported on the effect of spinal cord stimulation on the inflammatory response to atrial fibrillation.
OBJECTIVE: To investigate the effect of spinal cord stimulation on the serum levels of inflammatory factors in canine atrial fibrillation models.
METHODS: Nine adult beagles from the Experimental Animal Center of Kunming Medical University were selected as laboratory canines and divided into control group (n=3), atrial fibrillation group (n=3) and atrial fibrillation+spinal cord stimulation group (n=3) at random. The canine atrial fibrillation models were made by rapid right atrial pacing in the latter two groups. The spinal cord stimulators were implanted into the canine atrial fibrillation models in the atrial fibrillation+spinal cord stimulation group and had released stimulation for 12 weeks. The miniaturized insertable cardiac monitors were implanted into the canine atrial fibrillation models for monitoring AT/AF burden. Venous blood samples of the experimental beagles were extracted, in which the serum levels of inflammatory factors (C-reactive protein, hypersensitive C-reactive protein, interleukin-1, interleukin-6 and complement-3) were tested by ELISA. In the atrial fibrillation group, the blood samples were taken before and after modeling (before pacemaker implantation and when atrial fibrillation had lasted for 15 minutes), while in the atrial fibrillation+spinal cord stimulation group, the blood samples were extracted before and after spinal cord stimulation therapy (before spinal cord stimulation therapy and when spinal cord stimulation had implemented for 12 weeks). Meanwhile, venous blood samples were taken from each beagle in the control group.
RESULTS AND CONCLUSION: The serum levels of inflammatory factors in the atrial fibrillation group were significantly higher than those in the control group (P < 0.05), while the levels were significantly decreased in the atrial fibrillation+spinal cord stimulation group compared to the atrial fibrillation group (P < 0.05). For the canine models of atrial fibrillation, the serum concentration of inflammatory factors in the beagles which had received spinal cord stimulation therapy for 12 weeks were all lower than those without spinal cord stimulation (P < 0.05). The AT/AF burden of the atrial fibrillation+spinal cord stimulation group declined significantly compared with the atrial fibrillation (P < 0.05). To conclude, atrial fibrillation can induce inflammatory response, and spinal cord stimulation can suppress atrial fibrillation and further inhibit the inflammatory response caused by atrial fibrillation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Atrial Fibrillation, Spinal Cord, Electric Stimulation, Models, Animal, Tissue Engineering

CLC Number:

R454.1
R364

Shi Hui, Zhao Lulu, Hua Baotong, Du Yunhui, Guo Tao. Spinal cord stimulation alters the serum levels of inflammatory factors in canine atrial fibrillation models[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1023-1029.

Figures/Tables 8

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