Evaluation of lower limb function in rat models of spinal cord injury at different segments

doi:10.3969/j.issn.2095-4344.1058

Abstract

Abstract:

BACKGROUND: An ideal animal model is essential for studies on the pathophysiologic and anatomical structures. Therefore, selecting injury method and a safe and effective segment of spinal cord is critical.
OBJECTIVE: To explore the effects of different spinal cord injury segments on the recovery of lower extremity function.
METHODS: Rat models of spinal cord injury at different segments were established. Seventy-two Sprague-Dawley rats were randomly divided into six groups: T8, T9, T10, T11, T12 and sham operation (laminectomy and spinal meninge exposure) groups. The rats in the T8, T9, T10, T11, T12 groups were used for establishing rat models of spinal cord injury by clipping the corresponding spinal cord segments using a microvessel clamp.
RESULTS AND CONCLUSION: In the climbing grid experiment, the number of rats stepping on the air in each group decreased with time. Compared with the sham operation group, the Basso, Beattie, and Bresnahan scores in each group were significantly decreased. At 2-4 weeks after injury, the Basso, Beattie, and Bresnahan scores in the T9, T10 and T11 groups were higher than those in the T8 and T12 groups, and the scores in the T10 group were higher than those in the T9 and T11 groups. In summary, T10 group showed the best recovery of lower limb function after spinal cord injury.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Spinal Cord Injuries, Models, Animal, Tissue Engineering

CLC Number:

R441
R741

Wei Weibing1, Zhou Xiangxing1, Zhou Binbin2, Li Yijun1, Zou Zhilong1, Yang Hui1 . Evaluation of lower limb function in rat models of spinal cord injury at different segments[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1073-1077.

Figures/Tables 3

2.1 实验动物数量分析 T8，T9，T12组分别死亡了1只大鼠，及时补充基线一致的大鼠。最终有72只大鼠进入结果分析。 2.2 大体情况脊髓损伤后大鼠的下肢运动功能丧失，经针刺钳夹等刺激未见反应，同时出现脊髓损伤后的便障碍及尿潴留的症状，造模后前3 d部分大鼠出现蛋白尿、甚至血尿的情况，7 d后大鼠基本可以自主排便。 2.3 下肢功能 2.3.1 爬网格实验结果造模后第3天时，假手术组大鼠有少量踏空现象，T8组、T9组、T10组、T11组、T12组大鼠均靠前肢的力量牵引其到达网格顶端；至第7天假手术组基本可以支持爬向网格顶端，其余组大鼠可见有下肢少量的网格支撑；随着时间的推移，假手术组几乎可以正常爬行，其余各伤组大鼠的踏空次数逐渐减少。不同时间段各组大鼠的爬网格实验踏空次数见表1。 2.3.2 BBB评分假手术组在造模后2 h下肢肌力受限，关节可活动但行动迟缓，1 d时大鼠的关节活动功能良好，但协调能力稍差四肢不能协调前行，3 d时下肢功能恢复良好，双侧下肢基本可以支撑协调前行。 T8组、T9组、T10组、T11组、T12组大鼠造模后2 h表现为截瘫，下肢功能丧失肌力为0，造模后第3，7，14，21，28天BBB评分明显低于假手术组(P ≤ 0.05)。造模后第3，7天时T8组、T9组、T10组、T11组、T12组BBB评分接近(P > 0.05)；造模后第14天，T9组、T10组、T11组大鼠BBB评分高于T8组和T12组，且T9组、T10组、T11组大鼠BBB评分接近(P > 0.05)；造模后第21天时T9组、T10组、T11组大鼠BBB评分高于T8组和T12组(P ≤ 0.05)，且T10组大鼠BBB评分高于T9组和T11组(P ≤ 0.05)，T9组与T11组大鼠BBB评分接近(P > 0.05)；造模后第28天时T9组、T10组、T11组大鼠BBB评分高于T8组和T12组(P ≤ 0.05)，且T10组大鼠BBB评分高于其他组(P ≤ 0.05)。不同时间段各组大鼠BBB评分见表2，图3。"

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