Dominant T cell and B cell epitopes in EgA31 protein of Echinococcus granulosus by bioinformatics

doi:10.3969/j.issn.2095-4344.1001

Abstract

Abstract:

BACKGROUND: EgA31 protein participates in acetabulum muscle contraction, and is an important candidate vaccine in the protective immunity of Echinococcus granulosus.
OBJECTIVE: To analyze the EgA31 protein of the Echinococcus granulosus using bioinformatics and to predict the potential dominant T cell and B cell epitopes.
METHODS: The amino acid sequence of EgA31 from the NCBI GenBank database was obtained. The molecular mass, theory isoelectric point, amino acid composition, atomic composition, extinction coefficient, instability coefficient, and total average hydrophobicity were analyzed using ProtParam online program. The secondary structure of EgA31 protein was analyzed using protein module of DNAstar software and SOPMA online services. The tertiary structure model of EgA31 protein was established Phyre server. Finally, the dominant T cell and B cell epitopes were predicted using ABCpred, BepiPred, SYFPEITHI, and IDBE software.
RESULTS AND CONCLUSION: EgA31 protein consisted of 601 amino acids, including 112 negatively charged residues and 121 positively charged residues, which were classified as the unstable and hydrophilic protein. Online service analysis revealed that the second structure of EgA31 protein comprised 82.36% of α-helixs, 4.16% of β-sheets, 3.16% of β-turns and 10.32% of random coils. According to ABCpred, BepiPred, SYFPEITHI and IDBE databases, four dominant T cell epitopes, six dominant T cell epitopes, and one T-B combined epitopes were predicted successfully. Bioinformatics can comprehensively predict the dominant T cell and B cell epitopes in EgA31 protein of the Echinococcus granulosus, which lays a foundation for developing vaccine and reagent in the future.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Echinococcosis, T-Lymphocytes, Epitopes, B-Lymphocyte, Protein Structure, Secondary, Protein Structure, Tertiary, Vaccines, Tissue Engineering

CLC Number:

R446
R318

Zhao Xiao1, Zhang Fengbo2, Wang Hongying1, Yan Fang3, An Mengting1, Li Yujiao1, Pang Nannan4, Ding Jianbing1. Dominant T cell and B cell epitopes in EgA31 protein of Echinococcus granulosus by bioinformatics[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1078-1083.

Figures/Tables 6

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