Preparation of bacterial cellulose dressing and its effect on accelerating wound healing

doi:10.3969/j.issn.2095-4344.0923

Abstract

Abstract:

BACKGROUND: There are various wound dressings with their own merits and demerits in the market. Increasing studies have confirmed the feasibility of bacterial cellulose as wound dressing, while few reports has been published to compare bacterial cellulose with other kinds of wound dressings in promoting wound healing.

OBJECTIVE: To study the wound healing effect of bacterial cellulose wound dressing (BC-HEC-1) in comparison with gauze and hydrocolloid in rats.

METHODS: BC-HEC-1 was sterilely prepared by Gluconacetobacter xylinus fermenting followed by a series of process steps. The cytotoxicity, moisture content and pH were examined. In addition, the component and surface morphology of BC-HEC-1 were examined by infrared spectroscopy and scanning electron microscope respectively. Sprague-Dawley rats were anesthetized and a 2 cm×2 cm skin wound was made in each rat by sterile surgery. All the animals were thereafter divided into three groups (n=20 per group): gauze group, hydrocolloid group and BC-HEC-1 group, wrapped with the corresponding dressing, respectively. The dressing in each group was changed every day. Wound area was calculated by measuring wound length and width every other days after surgery. Wound samples from each rat were taken for histological observation at 4, 7, 14, 21 days after surgery. Meanwhile, serum levels of transforming growth factor beta 1 and platelet-derived growth factor were detected.

RESULTS AND CONCLUSION: BC-HEC-1 was proved to have good hydrophilic property and no cytotoxicity. The water content was between 95%-98% and the pH was 6.5. The structure of BC-HEC-1 was identified by the infrared spectrum. Scanning electron microscope results showed that BC-HEC-1 is a nanoscale biomaterial. Compared with the traditional gauze dressing and hydrocolloid dressing, BC-HEC-1 wound dressing significantly increased the wound healing rate at 4-14 days after surgery (P < 0.05), and showed better moisturizing and anti-sticking effect as well as smaller wound area. The wound healing rate in the BC-HEC-1 group was significantly higher than that in the gauze and hydrocolloid groups at 4-14 days after surgery. The serum level of platelet-derived growth factor in the BC-HEC-1 group was also significantly increased compared with the gauze and hydrocolloid groups at 7 days after surgery (P < 0.05). Histopathological observation indicated BC-HEC-1 could promote the granulation tissue growth moderately and orderly, while the granulation tissues in the other groups had relative thin thickness, loose structure, and irregular arrangement. In conclusion, the BC-HEC-1 can effectively promote wound healing, and meanwhile, it shows better moisturizing effects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Biocompatible Materials, Transforming Growth Factor beta1, Platelet-Derived Growth Factor

CLC Number:

R318

Xu Ling-hua, Jiang Xiao-mei, Bao Su-min, Hu Xue-feng, Liu Quan, Wu Bo, Xiao Ying, Li Jing. Preparation of bacterial cellulose dressing and its effect on accelerating wound healing[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(26): 4150-4155.

Figures/Tables 8

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