Fibroblasts-derived induced pluripotent stem cells in pulp revascularization: differentiation into vascular endothelial cells and co-culture with dental pulp stem cells

doi:10.3969/j.issn.2095-4344.0902

Abstract

Abstract:

BACKGROUND: Dental pulp vascularization is an important process to provide nutrients and energy for the dental pulp regeneration. The choice of stem cells and their directional differentiation into vascular endothelial cells have become an important issue of research in the pulp regeneration.
OBJECTIVE: To investigate the feasibility of mouse fibroblasts-derived induced pluripotent stem cells differentiating into vascular endothelial cells and forming new blood vessels by co-culture with dental pulp stem cells.
METHODS: (1) The feeder layer of mouse Sertoli cells was prepared by enzyme digestion. (2) Mouse fibroblasts-derived induced pluripotent stem cells were induced by vascular endothelial growth factor and basic fibroblast growth factor to differentiate into vascular endothelial cells, and differentiated cells were then identified. (3) The co-culture system of vascular endothelial cells and dental pulp stem cells was established. Real-time fluorescence quantitative real-time PCR was used to detect mRNA expression of CD34 and vascular endothelial growth factor in the two groups.
RESULTS AND CONCLUSION: After 14 days of induced differentiation, the morphology of the vascular endothelium was visible under the inverted microscope. Flow cytometry results showed the differentiated cells were positive for CD31, indicating the phenotype of endothelial cells. Compared with the control group, the expression of endothelin and endothelial nitric oxide synthase increased (P < 0.01), and the expression of prostacyclin decreased in the co-culture group at 14 days of induced differentiation (P < 0.01). Compared with the control group, the expression of CD34 and vascular endothelial growth factor increased at 5 days of co-culture (P < 0.01). These findings indicate that mouse fibroblasts-derived induced pluripotent stem cells can directionally differentiate into vascular endothelial cells and form new blood vessels by co-culture with dental pulp stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Induced Pluripotent Stem Cells, Endothelial Cells, Dental Pulp, Stem Cells, Tissue Engineering

CLC Number:

R394.2

Lü Ji-zhong. Fibroblasts-derived induced pluripotent stem cells in pulp revascularization: differentiation into vascular endothelial cells and co-culture with dental pulp stem cells[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(21): 3371-3375.

Figures/Tables 1

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