Expression of toll-like receptor 4/nuclear factor-kappa Bp65 signaling pathway in rat myocardium after endurance exercise

doi:10.3969/j.issn.2095-4344.0798

Abstract

Abstract:

BACKGROUND: Acute myocardial ischemia and hypoxia can upregulate the expression of angiotensin II, toll-like receptor 4 (TLR4) and nuclear factor κB (NFκB). In TLR4 deficient mice, cardiac hypertrophy is found to be decreased in the presence of pressure overload, suggesting that TLR4/NFκBp65 is a key signaling pathway in the development and progression of cardiac hypertrophy.
OBJECTIVE: To investigate the effect of TLR4/NFκBp65 signaling pathway activation on myocardial adaptation in rats subjected to graded endurance training of different exercise protocols.
METHODS: Rats were randomly divided into three groups: a single bout of exercise, 4- and 10-week endurance training, respectively. Treadmill was used for exercise training. All three groups were subdivided into: control group and exercise groups euthanized 0, 3, and 24 hours after last training.
RESULTS AND CONCLUSION: The expression level of TLR4 was sustained at high level after a single bout of exercise, but the expression level was increased only immediately after last training in 4 and 10 weeks of endurance in rats. The expression level of NFκBp65 showed a sustained increase in 4-week trained rats, but only transiently increased in 10-week trained rats after last run, and the expression was reduced in the 24-hour exercise group when compared with age-matched control group. The expression level of myosin heavy chain1/2/4/6 appeared to be continuously increased after a single bout of exercise and 4 weeks of endurance training, but was only transiently increased after 10 weeks of endurance training. The exercise-induced activation of TLR4/NFκBp65 signaling pathway may participate in the myocardial adaptation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Myocardium, Sports, Tissue Engineering

CLC Number:

R318

Gu Wei-yu, Liu Xiao-ran, Wang Zhi-qiang, Wang Yun-hong. Expression of toll-like receptor 4/nuclear factor-kappa Bp65 signaling pathway in rat myocardium after endurance exercise[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3201-3206.

Figures/Tables 2

2.1 实验动物数量分析所有120只大鼠均进入实验结果分析。 2.2 心系数一次急性运动与4周耐力训练后，24 h组与安静对照组大鼠的心系数无差异(P > 0.05)。10周耐力训练后，与安静对照组相比，24 h组心系数显著升高(P < 0.05；表2)。 2.3 心肌TLR4蛋白表达水平一次急性运动后，0，3和24 h组大鼠心肌TLR4蛋白表达水平分别比安静对照组显著提高了78.3%、84.0%和40.0%(P < 0.01，P < 0.01， P < 0.01；图1A)；4周耐力训练后，3 h组大鼠心肌TLR4蛋白表达水平比24 h组提高33.7%(P < 0.01)，3和0 h组大鼠心肌TLR4蛋白表达水平与安静对照组无差异(图1B)。10周耐力训练后，0和3 h组大鼠心肌TLR4蛋白表达水平比24 h组提高了40.3%、45.8%(P < 0.01，P < 0.01)，3和0 h组大鼠心肌TLR4蛋白表达水平与安静对照组无差异(图1C)。 2.4 心肌NFκBp65蛋白表达水平一次急性运动后，0，3和24 h组大鼠心肌NFκBp65蛋白表达与安静对照组无差异(图2A)；4周耐力训练后，24 h组大鼠心肌NFκBp65蛋白表达分别比安静对照组、0和3 h组提高了22.8倍、7.9倍和68.3%(P < 0.01，P < 0.01，P < 0.05；图2B)。10周耐力训练后，0和3 h组与24 h组大鼠心肌NFκBp65蛋白表达相比分别提高了3.2倍和4.24倍(P < 0.01，P < 0.01)，但24 h组与安静对照组相比无显著差异(图2C)。 2.5 心肌MYH1/2/4/6蛋白表达水平一次急性运动后，0，3和24h组大鼠心肌MYH1/2/4/6蛋白表达分别比安静对照组提高了4.1倍、3.6倍和3.8倍(P < 0.01，P < 0.01， P < 0.01；图3A)。4周耐力训练后，24 h组大鼠心肌MYH1/2/4/6蛋白表达比安静对照组提高了5.1倍(P < 0.05)，3和0 h组大鼠心肌MYH1/2/4/6蛋白表达与安静对照组无差异(图3B)。10周耐力训练后，24 h组大鼠心肌MYH1/2/4/6蛋白表达比安静对照组提高了41.2%(P < 0.05)，3 h比24 h组提高了28.2% (P < 0.05)，3和0 h组大鼠心肌MYH1/2/4/6蛋白表达与安静对照组无差异(图3C)。 2.6 心肌病理变化苏木精-伊红染色结果显示，4周安静对照组心肌纤维排列整齐，胞浆染色均匀，无明显充血、水肿。耐力训练后24 h组胞浆淡染，排列不规整，局部有渗出。10周安静对照组的心肌细胞结构正常，且心肌纤维排列整齐均匀，耐力训练运动后24 h组可观察到心肌有充血现象，且局部肌纤维肿胀，心肌纤维增粗(图4)。"

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