Purification process of non-denatured type II collagen

doi:10.3969/j.issn.2095-4344.0641

Abstract

Abstract:

BACKGROUND: Increasing studies have been concentrated on the purification of non-denatured collagen at the laboratory level, but there are fewer reports on the extraction and purification of pilot scale and production scale. After all, this technology has to be out of the laboratory to make sense. At the trial and production stages, more is faced with the measurement of cost, yield, and ease of operation.

OBJECTIVE: To explore the methods and conditions for the desalination and deacidification purification of non-denatured type II collagen in the pilot test and production.

METHODS: The commonly used desalination and deacidification methods for non-denatured type II collagen extracted included dialysis bag method, water washing method, rejection filtration method, and hollow fiber membrane method. The denatured type II collagen was purified according to these four methods. Disadvantages and advantages of the dialysis bag method, water washing method, rejection filtration method, and hollow fiber membrane method in the collagen purification process were compared using three indexes: yield, collagen content, and purity.

RESULTS AND CONCLUSION: For the yield, the ranking order was as follows: dialysis bag method > hollow fiber membrane method > rejection filtration method > water washing method; for the collagen content, dialysis bag method > hollow fiber membrane method > water washing method > rejection filtration method; for the purity of collagen, there was no significant difference among the four methods. Moreover, pressure was positively but insignificantly correlated with the membrane flux. By comparing the yield, collagen content, collagen purity, pressure difference, and membrane flux numerical value, it is considered that the hollow fiber membrane method has a comparative advantage in the purification of non-denatured type II collagen. This concludes that the hollow fiber membrane method is more suitable for industrial production, which has been patented on May 1, 2013 with national patent No. ZL 2011 10065733.X, and the authors are the first inventors of the patent.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen Type II, Technology, Tissue Engineering

CLC Number:

R318

Li Jie, Wang Yumei. Purification process of non-denatured type II collagen[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(34): 5496-5500.

Figures/Tables 4

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