Macrophages promote the healing of severe diabetic foot wounds after tibial transverse transport

doi:10.3969/j.issn.2095-4344.0616

Abstract

Abstract:

BACKGROUND: Ilizarov biological theory, tension stress technology, produces a certain tension by biological tissue stretch that can induce tissue regeneration and growth.
OBJECTIVE: To explore the role of macrophages in promoting the healing of severe diabetic foot wounds by tibial transverse transport.
METHODS: Ten patients with severe diabetic foot (Wagner grades ≥ 3), admitted in the Department of Bone and Joint Surgery, the First Affiliated Hospital of Guangxi Medical University from December 2017 to January 2018 were enrolled. With the consent of the patients and the permission of the ethics committee, the tissue sections were obtained from the edge of the wound before and at 1 month after surgery. The immunohistochemical technique was used to mark the M1 macrophages with CD86 monoclonal antibody, and the M2 macrophages with CD163 monoclonal antibody, and the positive cells were counted in the high magnification field. The Image pro plus 6.0 software was used to collect and analyze the pixels in the image, and the change of M1/M2 ratio was calculated.
RESULTS AND CONCLUSION: The 1-month postoperative M1/M2 ratio was significantly lower than that of the preoperative patients (P < 0.05). Postoperative CD86-positive cells and CD163-positive cells were significantly decreased in 400-fold positive cells counting compared with preoperation (P < 0.05). These findings suggest that tibial transverse transport can turn the macrophages into M2 macrophages, reduce the inflammation of the chronic wound of diabetic foot mediated by macrophages, and promote wound healing.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Macrophages, Diabetic Foot, Wound Healing, Tissue Engineering

CLC Number:

R318

Gao Wei, Lin Zhenxun, Zhen Puxiang, Chen Yan, Kuang Xiaocong, Hua Qikai. Macrophages promote the healing of severe diabetic foot wounds after tibial transverse transport[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(36): 5811-5815.

Figures/Tables 2

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