Abstract:

BACKGROUND: Menopause can also lead to changes in lean tissue and fat composition. Osteoporosis is found to be more common in obese elderly than in non-obese people. Thereafter, leptin may be an important regulatory factor in the pathogenesis of osteoporosis.
OBJECTIVE: To investigate the effect of Bushen Jianpi Recipe on lipid metabolism and leptin in high fat diet rats with osteoporosis.
METHODS: Totally 220 SPF Sprague-Dawley rats aged 6 months were randomly divided into model group (n=170) and sham group (n=50). The model group underwent bilateral ovarian ligation and the sham group received the same intervention as the model group except that ovarian ligation was not performed. At 12 weeks after surgery, the rat models were randomized into normal, high-fat, high-fat plus Jiangu Granule (2 g/(kg•d)), and high-fat plus simvastatin (100 μg/(kg•d)) groups (n=40 per group). The administration began at 13 weeks after surgery via gavage. The normal and sham groups were given 2 mL of normal saline, once daily. After 2, 6, 12 and 24 weeks of treatment, the body mass was measured. The serum, lumbar vertebrae and proximal femurs were removed. The bone mineral density of the right tibia was measured by double energy X-ray. The concentration of serum osteocalcin, tartrate-resistant acid phosphatase isoform 5b (TRACP-5b), total cholesterol, triacylglycerol, leptin and leptin receptor were detected by ELISA. The mRNA expression of leptin and leptin receptor in bones was determined by qPCR.
RESULTS AND CONCLUSION: The rat body mass in the high-fat plus Jiangu Granule group was higher than that in the normal and high-fat groups at the same time, but lower than that in the sham and high-fat plus simvastatin groups. The expression levels of leptin and leptin receptor, and osteocalcin level in the high-fat plus Jiangu Granule group were significantly higher than those in the normal and high-fat groups, and the contents of TRACP-5b, cholesterol, and triacylglycerol were significantly lower than those in the Leptin and leptin receptor, the BGP concentration were higher than those in the normal diet model group and the high fat diet model group. The concentrations of TRACT-5b, cholesterol, and triglycerides were lower than those in the normal and high-fat groups (P < 0.05). These results indicate that Bushen Jianpi Recipe can regulate lipid metabolism disturbance, reduce blood viscosity and improve the structure of blood vessel wall. It can also increase the expression of leptin and leptin receptor in serum and bones of rats, improve bone metabolism, and prevent and treat primary osteoporosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drugs, Chinese Herbal, Osteoporosis, Leptin, Receptors, Leptin, Tissue Engineering