Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (28): 4463-4468.doi: 10.3969/j.issn.2095-4344.0389

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Stamping tensile test in rabbits: differences in mechanical parameters between veins and arteries

Zhang Yu-hao1, 2, Niu Pei1, Zhang Zhi-min2, Niu Xiao-long1, Shen Wen-zeng1, Zhou Yu-juan1, Liu Fu-lin2   

  1. 1Medical School of Hebei University, Baoding 071000, Hebei Province, China; 2Department of Cardiac Surgery, Affiliated Hospital of Hebei University, Baoding 071030, Hebei Province, China
  • Received:2018-06-06 Online:2018-10-08 Published:2018-10-08
  • Contact: Zhou Yu-juan, Master, Professor, Medical School of Hebei University, Baoding 071000, Hebei Province, China Corresponding author: Liu Fu-lin, Master, Chief physician, Professor, Master’s supervisor, Department of Cardiac Surgery, Affiliated Hospital of Hebei University, Baoding 071030, Hebei Province, China
  • About author:Zhang Yu-hao, Master candidate, Medical School of Hebei University, Baoding 071000, Hebei Province, China; Department of Cardiac Surgery, Affiliated Hospital of Hebei University, Baoding 071030, Hebei Province, China
  • Supported by:

    the Excellent Talent Project of Department of Finance of Hebei Province and Health and Family Planning Commission of Hebei in 2016, No. 361007

Abstract:

BACKGROUND: There are few studies on the graft patency and biomechanics following coronary artery bypass graft surgery.
OBJECTIVE: To analyze the differences in mechanical parameters between veins and arteries.
METHODS: Twenty rabbits were randomized into control and experimental groups (n=10 per group). One side of the rabbit external jugular veins in the experimental group and the rabbit carotid arteries in the control group were removed and stored into the preservation solution (no Ca+ solution) for stamping tensile test. The external diameter of veins and arteries under different pressures (0-26.6 kPa), active state (high K+ solution) and passive state was measured. The vascular morphology was observed by hematoxylin-eosin staining. The stamping tensile test results were analyzed.
RESULTS AND CONCLUSION: Under passive or active state, the vascular outer diameter (inner diameter)-pressure curve, vascular wall thickness-pressure curve and circumferential stretch ratio-pressure curve tended to be stable. The elasticity of the veins was significantly inferior to that of the arteries. After stamping, the outer diameter of veins began to increase, and was twice of the arteries finally. Stereomicroscopy observed that the venous wall was thinner. With the internal pressure increasing, the arterial and venous outer diameter tended to be stable at 13.3 and 5.32 kPa, respectively. The vessel cross-section results showed that the thickness of the veins was significantly smaller than that of the arteries. To conclude, with the intraluminal pressure increasing, venous elasticity peaked earlier, and its compliance was poor, so it was vulnerable to high pressure, which is in accordance with the thinner elastic layer of venous wall.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Veins, Elasticity, Coronary Artery Bypass, Off-Pump, Biomechanics, Tissue Engineering

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