Imaging characteristics of near-infrared fluorescent dye IR-780 in clear cell renal cell carcinoma

doi:10.3969/j.issn.2095-4344.0332

Abstract

Abstract:

BACKGROUND: The near-infrared fluorescent dyes that directly bind to tumor cells have been developed, and IR-780 stands out.
OBJECTIVE: To evaluate the specific imaging effect of near-infrared fluorescent dye IR-780 in clear cell renal cell carcinoma (ccRCC).
METHODS: Human ccRCC cells 786-O, ACHN and normal human embryonic kidney 293T cells in logarithmic growth phase were incubated with 20 μmol/L IR-780 for 30 minutes. The localization of the dye in ccRCC was observed by laser confocal microscopy. Human ccRCC cells 786-O in logarithmic phase was incubated with 20 μmol/L IR-780 for 30 minutes. The localization of the dye in mitochondria and lysosomes was observed under confocal laser scanning microscope. 10, 100, 1 000 and 10 000 DAPI-stained human ccRCC cells 786-O were respectively added to human blood samples. The mononuclear cells in each group were isolated and cultured with 20 μmol/L IR-780 for 30 minutes. DAPI and IR-780 double positive cells were observed under confocal laser scanning microscope.
RESULTS AND CONCLUSION: IR-780 possessed the ability to visualize a variety of ccRCC cells, but not for normal renal epithelial cells. IR-780 had obvious overlapping staining in lysosomes or mitochondria, indicating its selective accumulation in mitochondria and lysosomes in the organelles. IR-780 could detect a small amount of ccRCC in the blood. These results suggest that the near-infrared fluorescent dye IR-780 can specifically accumulate in ccRCC cells, which can be used for specific diagnosis of ccRCC.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fluorescent Dyes, Kidney Neoplasms, Molecular Imaging, Tissue Engineering

CLC Number:

R318

Zhang Wei1, Qu Zhi-yi1, Liu Xiao-yang1, Wang Fu-li2, Ye Yong-li2, Yan Fei2, Wei Di2, Yuan Jian-lin2. Imaging characteristics of near-infrared fluorescent dye IR-780 in clear cell renal cell carcinoma[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4532-4536.

Figures/Tables 2

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