Protective effects of probiotics on the cell membrane in different tissues of rats after treadmill exercise

doi:10.3969/j.issn.2095-4344.0206

Abstract

Abstract:

BACKGROUND: Changes in the immune function of different tissues after long-time exhaustive exercise stress and probiotics intervention are still unclear.
OBJECTIVE: To observe the morphological changes and cell membrane protective effects of different tissues and organs in rats after exhaustive exercise and probiotics intervention.
METHODS: Forty Sprague-Dawley male rats were randomly allocated into quiet control group, exercise control group, quiet administration group and exercise administration group. Rats in the exercise control and exercise administration groups underwent 6 weeks of exhaustive training, at a speed of 19.3 m/min, slope of 5o, once daily, 6 days weekly. Rats in the quiet administration and exercise administration groups were given 107 CFU/mL probiotics via gavage, 10.0 mL/(kg•d). The histopathological changes were observed under light microscope and Na+/K+-ATPase, Ca2+/Mg2+-ATPase and T-ATPase in the liver, intestine and stomach were tested after 6 weeks of training.
RESULTS AND CONCLUSION: After exhaustive exercise, the liver, stomach and intestinal tissue presented with structure disorders, swelling and inflammatory cell infiltration and an decrease in the activity of Na+/K+-ATPase, Ca2+/Mg2+-ATPase and T-ATPase. After probiotic solution administrated, the structure of liver, stomach and intestine arranged closely and clearly, with no obvious congestion and edema. However, there were a few inflammatory cells and slight ulcers, and the activities of Na+/K+-ATPase, Ca2+/Mg2+-ATPase and T-ATPase were increased. These findings show that probiotic solution can protect the liver, stomach and intestinal tissue from injury caused by long-time exhaustive exercise, and maintain the integrity and fluidity of cell membrane by increasing Na+/K+-ATPase, Ca2+/Mg2+-ATPase and T-ATPase activities.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Problotics, Sports Medicine, Adenosine Triphosphate, Tissue Engineering

CLC Number:

R318

Mo Wei-bin1, 2, Zhou Yan3, Yang Yan-tao1. Protective effects of probiotics on the cell membrane in different tissues of rats after treadmill exercise[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(12): 1877-1882.

Figures/Tables 2

2.1 实验动物数量分析实验选用大鼠40只，分为4组，实验过程无脱失，全部进入结果分析。 2.2 大鼠各组织的形态学变化比较 2.2.1 大鼠肝组织苏木精-伊红染色结果安静对照组和安静给药组大鼠肝脏细胞索清楚、肝窦、中央静脉细胞形态排列规则和汇管区细胞形态如常。运动对照组大鼠肝小叶结构明显紊乱、肿胀、胞质内有大量的脂滴和有炎症细胞浸润。运动给药组大鼠肝细胞空泡数目明显减少，但胞浆内的脂滴和炎症细胞浸润明显减少，但汇管区血管冲血，仍有少量的炎症细胞浸润。见图1。 2.2.2 大鼠胃组织苏木精-伊红染色结果光镜下观察发现，安静对照组和安静给药组大鼠胃组织细胞膜无发现破裂，胃黏膜连续性好，无炎性、无充血、水肿和糜烂等现象出现。运动对照组大鼠胃细胞膜层有大量的细胞破裂，胃黏膜层有出现溃疡、充血和水肿；运动给药组大鼠胃组织细胞膜层细胞破裂的比例明显减少，无明显的充血和水肿，但胃黏膜层还有轻微的溃疡。见图2。 2.2.3 大鼠肠组织苏木精-伊红染色结果通过光镜下观察发现，安静对照组和安静给药组大鼠小肠组织黏膜细胞没有破裂，绒毛上皮细胞排列紧密，黏膜组织结构层较清晰。运动对照组大鼠小肠组织黏膜细胞破裂，黏膜水肿、糜烂和充血，纤维结构紊乱；运动给药组大鼠小肠组织黏膜细胞破裂、坏死不明显，黏膜组织结构排列紧密、清晰。见图3。 2.3 大鼠各组织ATP酶代谢含量的比较分析见表1-3。由表1可知，运动对照组大鼠肝组织Na+/K+-ATP酶、Ca2+/Mg2+-ATP酶和T-ATP酶含量均低于安静对照组(P < 0.05); 安静给药组大鼠肝组织Na+/K+-ATP酶、Ca2+/Mg2+-ATP酶和T-ATP酶含量均高于运动对照组(P < 0.05)；运动给药组大鼠肝组织Na+/K+-ATP酶、Ca2+/Mg2+-ATP酶和T-ATP酶含量均高于运动对照组但低于安静对照组和安静给药组，其中运动给药组大鼠肝组织Na+/K+-ATP酶、Ca2+/Mg2+-TP酶与运动对照组比差异有显著性意义(P < 0.05)，运动给药组大鼠肝组织T-ATP酶与安静对照比较差异有显著性意义(P < 0.05)。由表2可知，运动对照组、运动给药组大鼠胃组织Na+/K+-ATP酶含量均低于安静对照组(P < 0.05或P < 0.01)，安静给药组与运动给药组大鼠胃组织Na+/K+-ATP酶含量均高于运动对照组(P < 0.01)；运动对照组大鼠胃组织Ca2+/Mg2+-ATP酶含量低于安静对照组(P < 0.01)，安静给药组大鼠胃组织Ca2+/Mg2+-ATP酶含量高于安静对照组和运动对照组(P < 0.01)；运动给药组大鼠胃组织Ca2+/Mg2+-ATP酶含量高于运动对照组(P < 0.01)；运动对照组大鼠胃组织T-ATP酶含量低于安静对照组(P < 0.01)，安静给药组与运动给药组大鼠T-ATP酶含量均高于运动对照组(P < 0.05或P < 0.01)。由表3可知，运动对照组和运动给药组大鼠肠组织Na+/K+-ATP酶含量低于安静对照组(P < 0.01)，安静给药组大鼠肠组织Na+/K+-ATP酶含量高于运动对照组(P < 0.01)，运动给药组大鼠Na+/K+-ATP酶含量高于运动对照组(P > 0.05)；运动对照组和运动给药组大鼠肠组织Ca2+/Mg2+-ATP酶含量低于安静对照组(P < 0.05)，安静给药组大鼠肠组织Ca2+/Mg2+-ATP酶含量高于运动对照组(P < 0.05)，运动给药组大鼠肠组织Ca2+/Mg2+-ATP酶含量高于运动对照组(P > 0.05)；运动对照组和运动给药组大鼠肠组织T-ATP酶含量低于安静对照组(P < 0.05)，安静给药组和运动给药组大鼠肠组织T-ATP酶含量高于运动对照组，其中安静给药组与运动对照组比较差异有显著性意义(P < 0.05)。"

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