Local administration of calcitonin gene-related peptide promotes early healing after rotator cuff injury   in mice

doi:10.3969/j.issn.2095-4344.0204

Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (12): 1859-1865.doi: 10.3969/j.issn.2095-4344.0204

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Local administration of calcitonin gene-related peptide promotes early healing after rotator cuff injury in mice

Chen Yang1, 2, 3, Wang Zhan-wen1, 2, 3, Chen Can1, 2, 3, Lv Hong-bin1, 2, 3

1Department of Sports Medicine and Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; 2Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; 3Xiangya Hospital of Central South University-International Chinese Musculoskeletal Research Society Collaborating Center for Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China

Received:2018-03-09 Online:2018-04-28 Published:2018-04-28
Contact: Lv Hong-bin, Professor, Chief physician, Doctoral supercisor, Postdoctoral supervisor, Department of Sports Medicine and Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; 2Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; 3Xiangya Hospital of Central South University-International Chinese Musculoskeletal Research Society Collaborating Center for Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
About author:Chen Yang, Master candidate, Department of Sports Medicine and Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; Xiangya Hospital of Central South University-International Chinese Musculoskeletal Research Society Collaborating Center for Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Supported by:
the National Natural Science Foundation of China, No. 81730068 and 81472072; the Postdoctoral Innovation Research Project of Central South University, No. 2016zzts532

Abstract

Abstract:

BACKGROUND: Repair of tendon interface is a difficulty in orthopedics and sports medicine, and the formation of new bone is conducive to its healing. Calcitonin gene-related peptide (CGRP) plays a critical role in tissue homeostasis and repair, but its effect on the bone-tendon interface repair is unknown.
OBJECTIVE: To investigate the effect of CGRP on the expression of osteocalcin and to evaluate the effect of CGRP on the early healing of rotator cuff injury in a mouse model.
METHODS: A mouse model of supraspinatus insertion-humerus injury was created. All model mice were then randomized into two groups, and given the injection of 5 nmol/kg CGRP (experimental group) or same volume of normal saline (control group) through the glenohumeral joint immediately after operation, thrice weekly, for 2 weeks. The mice were sacrificed at postoperative 4 and 6 weeks to remove the rotator cuff samples for hematoxylin-eosin staining and biomechanical test. The mRNA and protein expression levels of osteocalcin were surveyed by qRT-PCR, immunohistochemistry, and western blot assay.
RESULTS AND CONCLUSION: At postoperative 4 and 6 weeks, the mRNA and protein expression of osteocalcin in the experimental group were significantly higher than those in the control group (P < 0.05). Compared with the control group, there were more fibrocartilages in the experimental group at 4 weeks postoperatively, and more new bone formation in the experimental group at 6 weeks postoperatively. At 4 weeks postoperatively, failure load in the experimental group increased slightly, but it was not significantly different from that in the control group (P > 0.05); at 6 weeks postoperatively, failure load in the experimental group was significantly higher than that in the control group (P <0.05). To conclude, local injection of CGRP can up-regulate the expression of osteocalcin and the formation of new bone at the injury site, which can enhance early healing the injured rotator cuff.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Shoulder Joint, Models, Animal, Calcitonin Gene-Related Peptide, Osteocalcin

CLC Number:

R318

Chen Yang1, 2, 3, Wang Zhan-wen1, 2, 3, Chen Can1, 2, 3, Lv Hong-bin1, 2, 3. Local administration of calcitonin gene-related peptide promotes early healing after rotator cuff injury in mice[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(12): 1859-1865.

Figures/Tables 2

References

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Local administration of calcitonin gene-related peptide promotes early healing after rotator cuff injury in mice

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