Recombinant human bone morphogenetic protein 2 promotes osteogenic differentiation of bone marrow mesenchymal stem cells from intracapsular condylar fracture goats

doi:10.3969/j.issn.2095-4344.1820

Abstract

Abstract:

BACKGROUND: Intracapsular fracture of the condyle may lead to traumatic temporomandibular joint ankylosis, which is mainly related to massive bone formation. Herein, we evaluated the osteogenic potential of bone marrow mesenchymal stem cells after different types of intracapsular condylar fractures, to provide cell biology and molecular evidence for traumatic temporomandibular joint ankylosis.
OBJECTIVE: To induce the osteogenesis of bone marrow mesenchymal stem cells after different types of goat intracapsular condylar fractures in vitro by recombinant human bone morphogenetic protein-2.
METHODS: Bilateral mandibular condyles of 16 healthy goats were randomly divided into 4 groups: 8 goats were used to make models of type A intracapsular condylar fractures of the left condyle (group A) and models of type B intracapsular condylar fractures of the right condyle (group B), and the other 8 goats were used to make models of type C intracapsular condylar fractures of the left condyle (group C) and models of type D intracapsular condylar fractures of the right condyle (group D). One rat from each group was sacrificed at the following intervals, 1, 2, 3 and 6 months postoperatively. Passage 3 bone marrow mesenchymal stem cells were isolated, extracted and induced by recombinant human bone morphogenetic protein-2 (induction group) or not (non-induced group), and cultured according to the kit’s instruction. The alkaline phosphatase activity and osteocalcin content were measured at 7 and 10 days after induced culture, and alkaline phosphatase staining was performed at 1 week after induced culture.
RESULTS AND CONCLUSION: Compared with the corresponding non-induced group, the alkaline phosphatase activity and the osteocalcin content in groups A, B, C, and D after induction with recombinant human bone morphogenetic protein-2 were significantly increased (P < 0.05). The alkaline phosphatase activity and osteocalcin content in group B were significantly higher than those in groups A, C, and D after induced by recombinant human bone morphogenetic protein-2 (P < 0.05). To conclude, the use of recombinant human bone morphogenetic protein-2 can trigger marked osteoblast differentiation of goat bone marrow mesenchymal stem cells. Moreover, the osteogenic activity of bone marrow mesenchymal stem cells after type B intracapsular condylar fractures was superior to that after type A and C intracapsular condylar fractures.

Key words: intracapsular condylar fracture, bone marrow mesenchymal stem cells, recombinant human bone morphogenetic protein-2, alkaline phosphatase activity, osteocalcin, osteogenic differentiation, traumatic temporomandibular joint ankylosis, Natural Science Foundation of Xinjiang Uygur Autonomous Region

CLC Number:

Hu Juan, Yao Zhitao, Wang Shan, Huate•Hadeer, Maimaitituxun•Tuerdi. Recombinant human bone morphogenetic protein 2 promotes osteogenic differentiation of bone marrow mesenchymal stem cells from intracapsular condylar fracture goats[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(29): 4610-4616.

Figures/Tables 4

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