Cell biocompatibility of an acellular heart scaffold of Sprague-Dawley neonatal rats

doi:10.3969/j.issn.2095-4344.0016

Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (2): 261-266.doi: 10.3969/j.issn.2095-4344.0016

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Cell biocompatibility of an acellular heart scaffold of Sprague-Dawley neonatal rats

Wei Ya-shu¹, Xu Yi-chen², Zhao Wen-jing¹, Wang Hui-feng¹, Liu Hong-jing¹, Xie Dan-ni^{1, 2}, Chen Wei-ping¹

¹Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; ²Guangxi Zhuang Autonomous Region Family Planning Research Center, Nanning 530021, Guangxi Zhuang Autonomous Region, China

Received:2017-12-13 Online:2018-01-18 Published:2018-01-18
Contact: Xie Dan-ni, Chief physician, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Guangxi Zhuang Autonomous Region Family Planning Research Center, Nanning 530021, Guangxi Zhuang Autonomous Region, China Chen Wei-ping, Professor, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
About author:Wei Ya-shu, Studying for master’s degree, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Supported by:
the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2012GXNSFAA053126; the Funded Project of Guangxi Department of Science and Technology, No. 0832157, 0592007-1G; Emergency and Medical Rescue Talent Small Highland in Guangxi Zhuang Autonomous Region • Open Topics of Key Laboratory for Emergency Medicine in Guangxi Universities, No. GXJZ201505; the Science Foundation for the Youth in the Guangxi Medical University, No. GXMUYSF201528; 2017 Basic Ability Promotion Project for Young and Middle-Aged Teachers in Guangxi Universities, No. 2017KY0096; Open Project of Guangxi Key Laboratory of Regenerative Medicine, No. 201703; Research Foundation of the Population and Family Planning Commission of Guangxi Zhuang Autonomous Region, No. 1108

Abstract

Abstract:

BACKGROUND: Owing to the advantages of low sensitization and natural three-dimensional structure, good biocompatibility and cell affinity, acellular heart scaffold materials are of great current interest in cardiac tissue engineering.

OBJECTIVE: To investigate the cytocompatibility of an acellular heart scaffold of neonatal rats.

METHODS: In order to construct the seed cell-scaffold complex, passage 3 bone marrow mesenchymal stem cells (BMSCs) of Sprague-Dawley neonatal rats were cultured with an acellular heart scaffold of Sprague-Dawley neonatal rats for 7 and 14 days. Hematoxylin eosin staining and scanning electron microscopy were used to observe the growth of BMSCs in the scaffold. The cell-scaffold complex was induced in myocardial tissue lysate for 14 days. BMSCs with planar orientation differentiation for 14 and 20 days were used as control group. RT-PCR was used to detect the expression of myosin heavy chain α-MHC and zinc finger transcription factor GATA-4 in BMSCs.

RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining showed the acellular heart scaffold contained a large number of eosinophilic fibrous structures, and the cell number of cell-scaffold complex after co-culture for 14 days was higher than that after co-culture for 7 days. Under the scanning electron microscope, a large amount of cells adhered to the fiber surface of the acellular scaffold at 14 days of co-culture. (2) BMSCs with planar orientation differentiation for 14 and 20 days had the bamboo-like and myotube-like structures. In the cell-scaffold complex with planar orientation differentiation for 14 days, the expression of α-MHC and GATA-4 could be detected, and their expression levels fulfilled the requirement for the presence of bamboo-like cells and myotube-like structure. These results indicate that the acellular heart scaffold exhibits good cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Stem Cells, Myocytes, Cardiac, Myosin Heavy Chains, Tissue Engineering

CLC Number:

R318

Wei Ya-shu, Xu Yi-chen, Zhao Wen-jing, Wang Hui-feng, Liu Hong-jing, Xie Dan-ni, Chen Wei-ping.

Cell biocompatibility of an acellular heart scaffold of Sprague-Dawley neonatal rats

[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(2): 261-266.

Figures/Tables 4

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Cell biocompatibility of an acellular heart scaffold of Sprague-Dawley neonatal rats

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