Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4619-4622.doi: 10.3969/j.issn.1673-8225.2011.25.015

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Relative biological evaluation of hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly(lactide-co-glycolde) cultured with bone marrow mesenchymal stem cells

Tan Yu-ying1, Zhang Duo1, Li Yu-xin2, Sun Ying2, Xu Yang3, Li Chun1, Jin Hong-juan1, Shao Ying1   

  1. 1Department of Plastic and Reconstructive Surgery, First Hospital, Jilin University, Changchun  130021, Jilin Province, China
    2National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun  130024, Jilin Province, China
    3Department of Plastic and Reconstructive Surgery, Siming Branch of First Affiliated Hospital, Xiamen University, Xiamen  361002, Fujian Province, China
  • Received:2011-01-04 Revised:2011-04-15 Online:2011-06-18 Published:2014-01-10
  • Contact: Shao Ying, Doctor, Associate professor, Department of Plastic and Reconstructive Surgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, China lele_shao@163.com
  • About author:Tan Yu-ying★, Studying for master’s degree, Department of Plastic and Reconstructive Surgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, China tyy_316@163.com
  • Supported by:

    Science Frontiers and Cross-disciplinary Innovation Projects of Jilin University, No. 200903068*

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Abstract:

BACKGROUND: The hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly (lactide-co-glycolde) (PLGA/g-HA) composite material is preferable to good stability and favorable mechanical property. As scaffold cultured with bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To study the bioactivity and biocompatibility of BMSCs cultured with PLGA/g-HA composite material in vitro.
METHODS: Third passage BMSCs of rabbits were cultured in PLGA/g-HA extract at different concentrations (10%, 30%, 50% and 80%), and cell proliferation was detected by MTT method. Meanwhile, the cell adhesion and morphology were observed with scanning electron microscopy.
RESULTS AND CONCLUSION: There was no significant difference between 10% group and negative group at 1 and 3 days after culture. The cytotoxic grade was Grade 1 in all the four concentrations. BMSCs could stretch pseudopodium gradually and adherent on the surface of PLGA/g-HA firmly. The results indicated that: ①BMSCs could proliferate in PLGA/g-HA leaching liquor. ②BMSCs have a good state on the surface of PLGA/g-HA. And we can apply the scaffold to bone defect repair, because of its good biocompatibility and cellular adhesion.

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