Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4579-4582.doi: 10.3969/j.issn.1673-8225.2011.25.006

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Acellular dermal matrix prepared by NaOH-maceration for ventral hernia repair

Wang Jun1, Zhang Bao-liang1, Yuan Qing-xin1, Mi Li-guo2   

  1. 1Department of General Surgery, Tangshan Workers’ Hospital Affiliated to Hebei Medical University, Tangshan  063000, Hebei Province, China
    2Department of Anatomy, Hebei Medical University, Shijiazhuang  050017, Hebei Province, China
  • Received:2011-01-06 Revised:2011-05-04 Online:2011-06-18 Published:2014-01-10
  • Contact: Mi Li-guo, Professor, Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
  • About author:Wang Jun★, Master, Associate chief physician, Department of General Surgery, Tangshan Workers’ Hospital Affiliated to Hebei Medical University, Tangshan 063000, Hebei Province, China junw1971@yahoo.cn
  • Supported by:

    Science and Technology Foundation of Hebei Health Bureau, No. 04106*

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Abstract:

BACKGROUND: The use of an acellular dermal matrix (ADM) may represent a new alternative to prosthetic mesh products.
OBJECTIVE: To study the value of porcine ADM made by NaOH-maceration as ventral hernia-repairing material.
METHODS: ADM was made from the full thick skin of the back of swines by NaOH-maceration. Forty-five male SD rats were randomly divided into three groups including ventral hernia group (n=7), ADM group (the ventral hernia repaired by 3.5 cm× 4.0 cm ADM, n=28) and Marlex mesh group (the ventral hernia repaired by 3.5 cm×4.0 cm Marlex mesh, n=10). The occurrence of ventral hernia was observed postoperatively 1 week. To evaluate ADM histology, 4 rats in ADM group were sacrificed at 1, 2, 3, 5 and 10 weeks after surgery respectively; 6 rats were respectively selected from the Marlex group and ADM group for the experiment of tension resistance at 5 weeks after surgery.
RESULTS AND CONCLUSION: The occurrence of ventral hernia in ADM group and Marlex mesh group was significantly lower than that of ventral hernia group 1 week after surgery (P < 0.001), but there was no difference between ADM group and Marlex mesh group(P > 0.05). The collagen of ADM did not change much after implantation. ADMs became infiltrated with little fibroblasts by 1 week after surgery. New wessels in ADMs were found by 2 weeks after surgery. The vessel density of ADMs kept stable by 5 weeks after surgery. The Marlex had a significantly higher breaking strength than that of the ADM (P < 0.001). But 5 weeks after surgery, the tension resistance of ADM-fascial interface was higher than that of the Marlex-fascial interface (P < 0.05). ADM prepared by the maceration of NaOH can be used as the material repairing ventral hernia.

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