Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4572-4574.doi: 10.3969/j.issn.1673-8225.2011.25.004

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Mass transfer characteristics of hemoglobin solution in ultrafiltration process

Xiao Ke, Liu Feng, Gao Wei, Wang Xiang, Wang Ying   

  1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing  400030, China
  • Received:2010-11-28 Revised:2011-01-17 Online:2011-06-18 Published:2014-01-10
  • Contact: Wang Xiang, Professor, Doctoral supervisor, Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China xwangchn@vipsina.com
  • About author:Xiao Ke★, Master, Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China Xiaoke83@yahoo.cn
  • Supported by:

    National Natural Science Foundation of China, No. 10572159*; the Science and Technology Planning Projects of Chongqing Science and Technology Committee, No. 2006ba5010*; Innovation and Indraught Program of Higher Education, No. B06023*; Open Foundation of Large Instruments and Equipment of Chongqing University, No. 2074920060080*

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Abstract:

BACKGROUND: Ultrafiltration membrane has the characteristics of little destruction of the natural conformation of the protein and high recovery, which has been used in the isolation and purification of protein.
OBJECTIVE: To optimize utrafiltration of hemoglobin by using of the mass transfer model in order to isolate highly purified hemoglobin.
METHODS: Penetration flux, concentration of solution and the relationship between pressure and flux of enrichment hemoglobin solution in the process of ultrafiltration were studied by membrane ultrafiltration isolation and purification methods. Membrane resistance and concentration polarization resistance were quantitatively described and the mass transfer model was established.
RESULTS AND CONCLUSION: ①For the solution concentration of 1.18, 1.30, 1.39, 1.56 g/L, the ultrafiltration flux was   1.937 5, 1.875 0, 1.812 4, 1.750 0 L/(m2•h) respectively at the pressure of 0.04 MPa, 12 ℃;  ②For the different pressure: 0.02, 0.03, 0.04, 0.05, 0.06 MPa, the ultrafiltration flux was 0.165, 0.245, 0.325, 0.400, 0.475 L/(m2•h) respectively with the same time; ③The membrane resistance was 0.118 2, and the pressure coefficient was 0.128. However, the gel layer was gradually formed with the increase of protein concentration; it was hard to concentrate the hemoglobin when the concentration was equal to the gel concentration. The gel concentration Cg was 18.82 g/L, and the ultrafiltration mass transfer coefficient k was 0.700 8.

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