Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4561-4564.doi: 10.3969/j.issn.1673-8225.2011.25.001

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Osteogenesis and vascularization of bone marrow mesenchymal stem cells transfected by pcDNA3/hVEGF165 combined with freeze-dried cancellous bone in vivo

Zhang Peng1, Dong Ling2, Yang Lian-jia3   

  1. 1Department of Orthopaedics, Urumqi General Hospital of Lanzhou Military Command, Urumqi  830000, Xinjiang Uygur Autonomous Region, China; 2Department of Physiology, the Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China; 3Department of Pathology, School of Stomatology, Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China
  • Received:2011-03-09 Revised:2011-05-06 Online:2011-06-18 Published:2014-01-10
  • About author:Zhang Peng☆, Studying for doctorate, Attending physician, Department of Orthopaedics, Urumqi General Hospital of Lanzhou Military Command, Urumqi 830000, Xinjiang Uygur Autonomous Region, China Pengzhang171@hotmail.com

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Abstract:

BACKGROUND: Previous studies have shown that vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) can improve the implant livability and growth.
OBJECTIVE: To study the effect of freeze-dried cancellous bone on the osteogenesis and vascularization of bone marrow stem cells transfected with pcDNA3/hVEGF165 in vivo.
METHODS: The rabbit bone marrow stem cells, which were transfected with pcDNA3/hVEGF165 by liposome mediated method and then adhered to freeze-dried cancellous bone, were implanted in the muscle pouches of rabbits. The rabbits were divided into three groups: freeze-dried cancellous bone group (A group), freeze-dried cancellous bone combined with bone marrow stem cells group (B group), freeze-dried cancellous bone combined with bone marrow stem cells transfected with VEGF group (C group).
RESULTS AND CONCLUSION: At the 8th week after implantation, it was found, compared with A group and B group, C group grew a large number of osteoblasts, osteoclasts and cartilage, and the number of vessels in C group was more than that in A or B group. The osteogenesis of the freeze-dried cancellous bone combined with bone marrow stem cells transfected with VEGF using pcDNA3/hVEGF165 by liposome mediated method is better than the freeze-dried cancellous bone or freeze-dried cancellous bone combined with bone marrow stem cells.

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