Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2577-2582.doi: 10.3969/j.issn.1673-8225.2011.14.023

Previous Articles     Next Articles

Inhibitory effect of bone marrow mesenchymal stem cells carrying cytosine deaminase gene on glioma cells in vitro

Xing Qi1, Song Fei2, Liu Jian2, Ji Guang-chun2, Zhang Da-qing2, Ma Yu-fang3   

  1. 1Department of Anesthesia, First Hospital Affiliated to Dalian Medical University, Dalian 116011, Liaoning Province, China
    2Department of Neurosurgery, Second Hospital Affiliated to Dalian Medical University, Dalian  116027, Liaoning Province, China
    3Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, Liaoning Province, China
  • Received:2010-09-26 Revised:2010-12-03 Online:2011-04-02 Published:2013-11-02
  • Contact: Song Fei, Doctor, Professor, Department of Neurosurgery, Second Hospital Affiliated to Dalian Medical University, Dalian 116027, Liaoning Province, China songfei168@163.com
  • About author:Xing Qi★, Master, Professor, Department of Anesthesia, First Hospital Affiliated to Dalian Medical University, Dalian 116011, Liaoning Province, China
  • Supported by:

    the Scientific Research of Education Committee of Liaoning Province in 2008, No. 2008Z081*; the Scientific Technology Foundation of Dalian City, No. 2008E13SF203*; the Natural Science Foundation of Liaoning Province in 2009, No. 20092165*

PDF

569

Abstract:

BACKGROUND: The bone marrow mesenchymal stem cells (BMSCs) expressing foreign cytosine deaminase gene (gene CD) can effectively transform 5-fluorouracil (5-FC) that was used before chemotherapy into chemotherapeutics 5-FC with cytotoxicity. BMSCs expressing gene CD significantly inhibited the growth of glioma cells in vitro.
OBJECTIVE: To explore the effects of BMSCs as gene therapy vector expressing exogenous gene cytosine deaminase gene on the proliferation of glioma C6 cells.
METHODS: Mouse MSCs were isolated and cultured. Lentivirus vector combined with cytosine deaminase gene and green fluorescent protein (GFP) was constructed. Using lentivirus packaging, cytosine deaminase gene and GFP were transferred to mouse BMSCs. BMSCs expressing cytosine deaminase gene and GFP were stably obtained and cocultured with glioma C6 cells. Following 5-FC was added to the medium, effects of cytosine deaminase gene on the proliferation of glioma cells were detected using flow cytometry.
RESULTS AND CONCLUSION: Lentivirus-mediated cytosine deaminase gene and GFP gene successfully transfected mouse BMSCs and formed C57BL/6 mMSC-codA/eGFP cells. C57BL/6 mMSC-codA/eGFP could induce obvious apoptosis of glioma C6 cells following treatment with 5-FC. The apoptotic rate of C6 glioma cells was 60% (P < 0.05) under the action of 5-FC at a concentration of 1×106 μg/L. It was thus concluded that C57BL/6 mMSC-codA/eGFP could convert 5-FC to 5-FU and had significant restriction, even fatal, influence on the growth of C6 glioma cells.

CLC Number: