Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2562-2566.doi: 10.3969/j.issn.1673-8225.2011.14.020

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Stereotactic transplantation of bone marrow mesenchymal stem cells for learning and memory ability of vascular rats

Li Li-jie1, Kong Fan-ming2, Zhang Xue-bin2, Wang Xin-ping3, Wang Shi-min3, Tang Fan2, Zhang Wen-zhi4, Su Xin4   

  1. 1Postgraduate College, Tianjin Medical University, Tianjin  300070, China; 2Department of Pathology, 3Department of Neurology, 4Cell Room of Institute of Neurosurgery, Huanhu Hospital, Tianjin  300060, China
  • Received:2010-10-11 Revised:2010-11-15 Online:2011-04-02 Published:2013-11-02
  • Contact: Wang Shi-min, Professor, Master’s supervisor, Department of Neurology, Huanhu Hospital, Tianjin 300060, China wangxinpingtj@sina.com
  • About author:Li Li-jie★, Studying for master’s degree, Postgraduate College, Tianjin Medical University, Tianjin 300070, China 976038027@qq.com
  • Supported by:

    Science and Technology Foundation of Tianjin Health Bureau*

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Abstract:

BACKGROUND: The key of stem cells for treating nervous tissue injury is the transplantation of stem cells that have regeneration capacity. The damaged neurons are restored by multiple action mechanisms, which recover the nervous function.
OBJECTIVE: To explore the effects of bone marrow mesenchymal stem cells (BMSCs) on learning and memory disorders of vascular dementia model rats.
METHODS: BMSCs were separated with density gradient centrifugation and screening the cells adherent to the plastic surface.
10 mg/L BrdU was used to label before cell transplantation. Adult female Wistar rats were used to establish vascular dementia rat models by Permanent bilateral carotid artery ligation, and they were then randomly divided into control group, saline group and transplantation group. In the transplantation group, BMSCs were transplanted into the damaged spinal cord by stereotaxis at day 7 following damage. In the saline group, an equal volume of saline was utilized. In the control group, the rats were left intact. In the transplantation group, BMSCs were transplanted into the damaged Hippocampus by stereotaxis at day 14 following damage. In the saline group, an equal volume of saline was utilized. In the control group, the rats were left intact. Spatial learning and memory was detected by the Morris Water maze. Rats were sacrificed at day 90. Brdu+neuron specific enolase, Brdu+glial fibrillary acidic protein (GFAP) immunohistochemistry double-staining cells, and the migration of Doublecortin (DCX, the marker of N Ps) from the subventricular zone to the damaged hippocampus were observed.
RESULTS AND CONCLUSION: ①Spatial learning and memory in rats (escape latency and the number of cross platform) was better in the transplantation group than in the control group (P < 0.05). No significant difference was determined in the control group and the saline group. ②Double-staining cells of immunohistochemistry could be found at the center of damage site and the surrounding site in rats of the transplantation group. Results indicated that BMSC transplantation can improve the recovery of nervous function of rats with spinal cord injury. Its mechanism may be correlated with the differentiation of transplanted cells into neuron-like and glial cell-like cells, secretion or promoting secretion of neurotrophic factors in host.

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